Structural basis for plant lutein biosynthesis from alpha-carotene
文献类型：期刊论文
| 作者 | Niu, Guoqi1,3; Guo, Qi4; Wang, Jia4; Zhao, Shun1; He, Yikun3; Liu, Lin5 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2020 |
| 卷号 | 117期号:25页码:14150-14157 |
| 关键词 | xanthophyll P450 hydroxylation chirality stereospecificity |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.2001806117 |
| 文献子类 | Article |
| 英文摘要 | Two cytochrome P450 enzymes, CYP97A3 and CYP97C1, catalyze hydroxylations of the beta- and epsilon-rings of alpha-carotene to produce lu- tein. Chirality is introduced at the C-3 atom of both rings, and the reactions are both pro-3 R-stereospecific. We determined the crys- tal structures of CYP97A3 in substrate -free and complex forms with a nonnatural substrate and the structure of CYP97C1 in a detergent -bound form. The structures of CYP97A3 in different states show the substrate channel and the structure of CYP97C1 bound with octylthioglucoside confirms the binding site for the carotenoid substrate. Biochemical assays confirm that the ferredoxin- NADP + reductase (FNR) -ferredoxin pair is used as the redox part- ner. Details of the pro -3 R stereospecificity are revealed in the retinal -bound CYP97A3 structure. Further analysis indicates that the CYP97B clan bears similarity to the beta-ring -specific CYP97A clan. Overall, our research describes the molecular basis for the last steps of lutein biosynthesis. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | WASHINGTON |
| WOS关键词 | CYTOCHROME P450 REDUCTASE ; ARABIDOPSIS-THALIANA ; STRUCTURE REFINEMENT ; CRYSTAL-STRUCTURE ; PROVIDES INSIGHT ; BETA-RING ; HYDROXYLASES ; BINDING ; METABOLISM ; EXPRESSION |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000546772500017 |
| 出版者 | NATL ACAD SCIENCES |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670794, 31530006] ; National Key R&D Program of China [2017YFA0503703] ; Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; Chang Jiang Scholars ProgramProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [Q2017241] ; Anhui Provincial Wanjiang Scholars Program |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21688]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 2.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10065 USA 3.Chinese Acad Sci, Inst Bot, Chinese Acad Sci Key Lab Photobiol, Beijing 100093, Peoples R China 4.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Guoqi,Guo, Qi,Wang, Jia,et al. Structural basis for plant lutein biosynthesis from alpha-carotene[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(25):14150-14157. |
| APA | Niu, Guoqi,Guo, Qi,Wang, Jia,Zhao, Shun,He, Yikun,&Liu, Lin.(2020).Structural basis for plant lutein biosynthesis from alpha-carotene.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(25),14150-14157. |
| MLA | Niu, Guoqi,et al."Structural basis for plant lutein biosynthesis from alpha-carotene".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.25(2020):14150-14157. |
入库方式： OAI收割
来源：植物研究所
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