Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis
文献类型：期刊论文
| 作者 | Huai, Junling; Jing, Yanjun; Lin, Rongcheng1,3
|
| 刊名 | PHYSIOLOGIA PLANTARUM
 |
| 出版日期 | 2020 |
| 卷号 | 169期号:3页码:369-379 |
| ISSN号 | 0031-9317 |
| DOI | 10.1111/ppl.13099 |
| 文献子类 | Article |
| 英文摘要 | Plants have evolved light signaling mechanisms to optimally adapt developmental patterns to the ambient light environments. CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and LONG HYPOCOTYL5 (HY5) are two critical components in the light signaling pathway in Arabidopsis thaliana. COP1 acts as an E3 ubiquitin ligase that targets positive regulators, such as HY5, leading to their degradation in darkness. However, functional analysis of the COP1-HY5 module in maize (Zea mays) has not been reported. Here, we investigated the expression patterns and roles of the COP1 and HY5 orthologs, ZmCOP1 and ZmHY5, in regulating photomorphogenesis. These two genes have high amino acid identities with their Arabidopsis homolog and were both regulated by light. Subcellular localization assay showed that ZmCOP1 was distributed in the cytosol and ZmHY5 localized in the nucleus. Exogenous expression of ZmCOP1 rescued the physiological defects of the cop1-4 mutant, and expression of ZmHY5 complemented the long hypocotyl phenotype of the hy5-215 mutant in Arabidopsis. Yeast two-hybrid and fluorescence resonance energy transfer assays showed that ZmCOP1 interacted with ZmHY5. Our study gains insight into the conserved function and regulatory mechanism of the COP1-HY5 signaling pathway in maize and Arabidopsis. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1399-3054 |
| WOS关键词 | TRANSCRIPTION FACTOR HY5 ; GENE-EXPRESSION ; COP1 GENE ; FINGER MOTIF ; BZIP PROTEIN ; PHYTOCHROME ; BIOSYNTHESIS ; ROLES ; TRANSDUCTION ; INTERACTS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000523269500001 |
| 出版者 | WILEY |
| 资助机构 | National Key Research and Development Program of China [2016YFD0100405] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009-003] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31800235] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21831]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huai, Junling,Jing, Yanjun,Lin, Rongcheng. Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis[J]. PHYSIOLOGIA PLANTARUM,2020,169(3):369-379. |
| APA | Huai, Junling,Jing, Yanjun,&Lin, Rongcheng.(2020).Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis.PHYSIOLOGIA PLANTARUM,169(3),369-379. |
| MLA | Huai, Junling,et al."Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis".PHYSIOLOGIA PLANTARUM 169.3(2020):369-379. |
入库方式： OAI收割
来源：植物研究所
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