Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting
文献类型：期刊论文
| 作者 | Ouyang, Min4; Li, Xiaoyi; Zhang, Jing6; Feng, Peiqiang; Pu, Hua; Kong, Lingxi6; Bai, Zechen6; Rong, Liwei6; Xu, Xiumei5; Chi, Wei6 |
| 刊名 | CELL
 |
| 出版日期 | 2020 |
| 卷号 | 180期号:6页码:1144-1159 |
| ISSN号 | 0092-8674 |
| DOI | 10.1016/j.cell.2020.02.045 |
| 文献子类 | Article |
| 英文摘要 | In eukaryotic cells, organelle biogenesis is pivotal for cellular function and cell survival. Chloroplasts are unique organelles with a complex internal membrane network. The mechanisms of the migration of imported nuclear-encoded chloroplast proteins across the crowded stroma to thylakoid membranes are less understood. Here, we identified two Arabidopsis ankyrin-repeat proteins, STT1 and STT2, that specifically mediate sorting of chloroplast twin arginine translocation (cpTat) pathway proteins to thylakoid membranes. STT1 and STT2 form a unique heterodimer through interaction of their C-terminal ankyrin domains. Binding of cpTat substrate by N-terminal intrinsically disordered regions of STT complex induces liquid-liquid phase separation. The multivalent nature of STT oligomer is critical for phase separation. STT-Hcf106 interactions reverse phase separation and facilitate cargo targeting and translocation across thylakoid membranes. Thus, the formation of phase-separated droplets emerges as a novel mechanism of intra-chloroplast cargo sorting. Our findings highlight a conserved mechanism of phase separation in regulating organelle biogenesis. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1097-4172 |
| WOS关键词 | SIGNAL RECOGNITION PARTICLE ; PROTEIN-TRANSPORT ; PRECURSOR PROTEINS ; PEPTIDE-BINDING ; ANKYRIN REPEAT ; TRANSLOCATION ; SEPARATION ; IMPORT ; PLASTIDS ; FEATURES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000520925300012 |
| 出版者 | CELL PRESS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471267, 31130059] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150100] ; National Key Research and Development Program of China [2017YFA0503700] ; Outstanding Talents Fund of Henan University, China ; State Key Laboratory of Biocatalysis and Enzyme Engineering of Hubei University, China ; Youth Innovation Promotion Association CAS, China [2013059] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21851]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan 3.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 4.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, 85 Minglun St, Kaifeng 475001, Peoples R China 5.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 6.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 7.Chinese Acad Sci, State Key Lab Mol Dev Biol, Beijing 100086, Peoples R China 8.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Ouyang, Min,Li, Xiaoyi,Zhang, Jing,et al. Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting[J]. CELL,2020,180(6):1144-1159. |
| APA | Ouyang, Min.,Li, Xiaoyi.,Zhang, Jing.,Feng, Peiqiang.,Pu, Hua.,...&Zhang, Lixin.(2020).Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting.CELL,180(6),1144-1159. |
| MLA | Ouyang, Min,et al."Liquid-Liquid Phase Transition Drives Intra-chloroplast Cargo Sorting".CELL 180.6(2020):1144-1159. |
入库方式： OAI收割
来源：植物研究所
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