Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids
文献类型：期刊论文
| 作者 | Sun, Binwen3; Liu, Zheyi; Fang, Zheng3; Dong, Wei4; Yu, Yang1,5; Ye, Mingliang; Liu, Lin4; Wang, Hongda1,5; Wang, Fangjun3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2020 |
| 卷号 | 92期号:3页码:2770-2777 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.9b05048 |
| 文献子类 | Article |
| 英文摘要 | Proteomics emerges from the protein identification to protein functional elucidation, which depends to a large extent on the characterization of protein sequences. However, a large part of proteome sequences remains unannotated due to the limitation in proteolytic digestion by golden standard protease trypsin. Herein, we demonstrated that a cyanobacterial protease VAILase could specifically cleave at the short-chain aliphatic amino acids valine, alanine, leucine, isoleucine and threonine with cleavage specificity about 92% in total for proteomic analysis. The unique features of VAILase cleavage facilitate the characterization of most proteins and exhibit high complementarity to trypsin, and 22% of the covered sequences by VAILase are unique. VAILase can greatly improve the coverages of sequences with abundant aliphatic residues that are usually dark regions in conventional proteomic analysis, such as the transmembrane regions within anion exchanger 1 and photosystem II. |
| 学科主题 | Chemistry, Analytical |
| 出版地 | WASHINGTON |
| 电子版国际标准刊号 | 1520-6882 |
| WOS关键词 | INTEGRAL MEMBRANE-PROTEINS ; EVOLVING PHOTOSYSTEM-II ; CRYSTAL-STRUCTURE ; HHOA PROTEASE ; TRYPSIN ; THROUGHPUT ; ACCURACIES ; BAND-3 |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000511509400056 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Key R&D Program of China [2016YFF0200504] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91853101, 21675152] ; Original Innovation Project of CAS [ZDBSLY-SLH032] ; DICP [ZZBS201603] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21928]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 2.Univ Sci & Technol China, Hefei, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Binwen,Liu, Zheyi,Fang, Zheng,et al. Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids[J]. ANALYTICAL CHEMISTRY,2020,92(3):2770-2777. |
| APA | Sun, Binwen.,Liu, Zheyi.,Fang, Zheng.,Dong, Wei.,Yu, Yang.,...&Wang, Fangjun.(2020).Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids.ANALYTICAL CHEMISTRY,92(3),2770-2777. |
| MLA | Sun, Binwen,et al."Probing the Proteomics Dark Regions by VAILase Cleavage at Aliphatic Amino Acids".ANALYTICAL CHEMISTRY 92.3(2020):2770-2777. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。