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PPR-SMR protein SOT1 has RNA endonuclease activity

文献类型：期刊论文

作者Zhou, Wen1; Lu, Qingtao; Li, Qingwei1; Wang, Lei1; Ding, Shunhua; Zhang, Aihong; Wen, Xiaogang; Zhang, Lixin; Lu, Congming2
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2017
卷号114期号:8页码:E1554-E1563
关键词photosynthesis PPR-SMR protein RNA endonuclease rRNA biogenesis
ISSN号0027-8424
DOI10.3389/fpls.2017.02134
文献子类Article
英文摘要Numerous attempts have been made to identify and engineer sequence-specific RNA endonucleases, as these would allow for efficient RNA manipulation. However, no natural RNA endonuclease that recognizes RNA in a sequence-specific manner has been described to date. Here, we report that SUPPRESSOR OF THYLAKOID FORMATION 1 (SOT1), an Arabidopsis pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5' end of the chloroplast 23S-4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.
学科主题Plant Sciences
出版地WASHINGTON
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEIN ; SINGLE-STRANDED RNA ; STRUCTURAL BASIS ; MODULAR RECOGNITION ; CRYSTAL-STRUCTURE ; MINI-III ; DOMAIN ; MUTS2 ; TRANSLATION ; MATURATION
语种英语
WOS记录号WOS:000417788900003
出版者NATL ACAD SCIENCES
资助机构Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSWSMC003] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21969]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Wen,Lu, Qingtao,Li, Qingwei,et al. PPR-SMR protein SOT1 has RNA endonuclease activity[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(8):E1554-E1563.
APA Zhou, Wen.,Lu, Qingtao.,Li, Qingwei.,Wang, Lei.,Ding, Shunhua.,...&Lu, Congming.(2017).PPR-SMR protein SOT1 has RNA endonuclease activity.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(8),E1554-E1563.
MLA Zhou, Wen,et al."PPR-SMR protein SOT1 has RNA endonuclease activity".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.8(2017):E1554-E1563.

入库方式： OAI收割

来源：植物研究所

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