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The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis

文献类型：期刊论文

作者Wang, Lei1; Li, Qingwei1; Zhang, Aihong; Zhou, Wen1; Jiang, Rui1; Yang, Zhipan; Yang, Huixia; Qin, Xiaochun; Ding, Shunhua; Lu, Qingtao
刊名MOLECULAR PLANT
出版日期2017
卷号10期号:1页码:183-196
ISSN号1674-2052
关键词phytol phosphorylation VTE6 phylloquinone PSI stability
DOI10.1016/j.foodchem.2017.05.127
文献子类Article
英文摘要Phytyl-diphosphate, which provides phytyl moieties as a common substrate in both tocopherol and phylloquinone biosynthesis, derives from de novo isoprenoid biosynthesis or a salvage pathway via phytol phosphorylation. However, very little is known about the role and origin of the phytyl moiety for phylloquinone biosynthesis. Since VTE6, a phytyl-phosphate kinase, is a key enzyme for phytol phosphorylation, we characterized Arabidopsis vte6 mutants to gain insight into the roles of phytyl moieties in phylloquinone biosynthesis and of phylloquinone in photosystem I (PSI) biogenesis. The VTE6 knockout mutants vte6-1 and vte6-2 lacked detectable phylloquinone, whereas the phylloquinone content in the VTE6 knockdown mutant vte6-3 was 90% lower than that in wild-type. In vte6 mutants, PSI function was impaired and accumulation of the PSI complex was defective. The PSI core subunits PsaA/B were efficiently synthesized and assembled into the PSI complex in vte6-3. However, the degradation rate of PSI subunits in the assembled PSI complex was more rapid in vte6-3 than in wild-type. In vte6-3, PSI was more susceptible to high-light damage than in wild-type. Our results provide the first genetic evidence that the phytol phosphorylation pathway is essential for phylloquinone biosynthesis, and that phylloquinone is required for PSI complex stability.
学科主题Chemistry ; Food Science & Technology ; Nutrition & Dietetics
电子版国际标准刊号1752-9867
出版地CAMBRIDGE
WOS关键词ELECTRON-TRANSFER ; FOREIGN QUINONE ; TOCOPHEROL BIOSYNTHESIS ; CHLOROPLAST DEVELOPMENT ; ANGSTROM RESOLUTION ; PLASTOQUINONE-POOL ; THYLAKOID PROTEIN ; MUTANT REVEALS ; A(1) SITE ; VITAMIN-E
语种英语
出版者CELL PRESS
WOS记录号WOS:000406199900047
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370274] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Strategic Priority Research Program of the Chinese Academy Sciences [XDB17030100]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/22061]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
2.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Lei,Li, Qingwei,Zhang, Aihong,et al. The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis[J]. MOLECULAR PLANT,2017,10(1):183-196.
APA Wang, Lei.,Li, Qingwei.,Zhang, Aihong.,Zhou, Wen.,Jiang, Rui.,...&Lu, Congming.(2017).The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis.MOLECULAR PLANT,10(1),183-196.
MLA Wang, Lei,et al."The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis".MOLECULAR PLANT 10.1(2017):183-196.

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来源：植物研究所

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