Characterization of a heat responsive UDP: Flavonoid glucosyltransferase gene in tea plant (Camellia sinensis)
文献类型：期刊论文
| 作者 | Su, Xiaojia2; Wang, Wenzhao4; Xia, Tao4; Gao, Liping4; Shen, Guoan3; Pang, Yongzhen |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2018 |
| 卷号 | 13期号:11 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0207212 |
| 文献子类 | Article |
| 英文摘要 | Tea plant (Camellia sinensis) accumulates abundant flavonoid glycosides that are the major bioactive ingredients in tea. Biosynthesis of flavonoid glycosides are catalyzed by UDP-glucosyltransferases (UGTs) that are widely present in plants. Among one hundred and seventy-eight UGTs genes that we have previously identified in tea plant, few of them have been functionally characterized. In the present study, we further identified UGT73A17 gene that is responsible for the biosynthesis of a broad range of flavonoid glycosides. Sequence analysis revealed that the deduced UGT73A17 protein showed high identity with 7-O-glycosyltransferases at amino acid level and it was clustered into the clade containing several 7-O-glycosyltransferases from other plant species. Enzymatic assays revealed that the recombinant UGT73A17 protein (rUGT73A17) exhibited activity toward flavonols (kaempferol, quercetin, and myricetin), flavones (apigenin, luteolin, and tricetin), flavanone (naringenin), isoflavones (genistein) and epicatechin gallate, yielding 7-O-glucosides as the major in vitro products. In particular, rUGT73A17 displayed higher activity at high temperatures (eg. 50 degrees C) than at low temperatures, which was consistent with its relatively high expression level at high temperatures. Two amino acid substitutions at I296L and V466A improved the enzymatic activity of rUGT73A17. Our study demonstrated that UGT73A17 is responsible for the biosynthesis of a broad range of flavonoid glucosides, which is also involved in heat response and quality of tea plant. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | FUNCTIONAL-CHARACTERIZATION ; NATURAL VARIATION ; HEADING DATE ; GREEN TEA ; GLYCOSYLTRANSFERASES ; ANTHOCYANINS ; EXPRESSION ; CLONING ; TASTE |
| 语种 | 英语 |
| WOS记录号 | WOS:000451325700018 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305, 31570306] ; Major State Basic Research and Development Program of ChinaNational Basic Research Program of China [2014CB138703] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20320]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Inst Med Plant Dev, Beijing, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 3.Anhui Agr Univ, Sch Life Sci, Hefei, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Xiaojia,Wang, Wenzhao,Xia, Tao,et al. Characterization of a heat responsive UDP: Flavonoid glucosyltransferase gene in tea plant (Camellia sinensis)[J]. PLOS ONE,2018,13(11). |
| APA | Su, Xiaojia,Wang, Wenzhao,Xia, Tao,Gao, Liping,Shen, Guoan,&Pang, Yongzhen.(2018).Characterization of a heat responsive UDP: Flavonoid glucosyltransferase gene in tea plant (Camellia sinensis).PLOS ONE,13(11). |
| MLA | Su, Xiaojia,et al."Characterization of a heat responsive UDP: Flavonoid glucosyltransferase gene in tea plant (Camellia sinensis)".PLOS ONE 13.11(2018). |
入库方式： OAI收割
来源：植物研究所
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