Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells
文献类型：期刊论文
| 作者 | Chen, Tong; Ji, Dongchao1; Tian, Shiping1,3
|
| 刊名 | BMC PLANT BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 18 |
| 关键词 | Endosomal dynamics Microdomain Plasma membrane Variable-angle epifluorescence microscopy |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/s12870-018-1246-0 |
| 文献子类 | Article |
| 英文摘要 | Background: The assembly of protein complexes and compositional lipid patterning act together to endow cells with the plasticity required to maintain compositional heterogeneity with respect to individual proteins. Hence, the applications for imaging protein localization and dynamics require high accuracy, particularly at high spatio-temporal level. Results: We provided experimental data for the applications of Variable-Angle Epifluorescence Microscopy (VAEM) in dissecting protein dynamics in plant cells. The VAEM-based co-localization analysis took penetration depth and incident angle into consideration. Besides direct overlap of dual-color fluorescence signals, the co-localization analysis was carried out quantitatively in combination with the methodology for calculating puncta distance and protein proximity index. Besides, simultaneous VAEM tracking of cytoskeletal dynamics provided more insights into coordinated responses of actin filaments and microtubules. Moreover, lateral motility of membrane proteins was analyzed by calculating diffusion coefficients and kymograph analysis, which represented an alternative method for examining protein motility. Conclusion: The present study presented experimental evidence on illustrating the use of VAEM in tracking and dissecting protein dynamics, dissecting endosomal dynamics, cell structure assembly along with membrane microdomain and protein motility in intact plant cells. |
| 学科主题 | Plant Sciences |
| 出版地 | LONDON |
| WOS关键词 | SINGLE-PARTICLE TRACKING ; ANCHORED PROTEIN ; TIRF MICROSCOPY ; RAPID GROWTH ; ARABIDOPSIS ; ACTIN ; CLATHRIN ; ENDOCYTOSIS ; MICROTUBULES ; MICRODOMAINS |
| 语种 | 英语 |
| WOS记录号 | WOS:000427947800002 |
| 出版者 | BMC |
| 资助机构 | NSFCNational Natural Science Foundation of China (NSFC) [31530057, 31672210, 31670183] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015BAD16B01-3] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20386]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Nanxincun 20, Beijing 100093, Peoples R China 2.Minist Agr, Key Lab Postharvest Handling Fruits, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tong,Ji, Dongchao,Tian, Shiping. Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells[J]. BMC PLANT BIOLOGY,2018,18. |
| APA | Chen, Tong,Ji, Dongchao,&Tian, Shiping.(2018).Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells.BMC PLANT BIOLOGY,18. |
| MLA | Chen, Tong,et al."Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells".BMC PLANT BIOLOGY 18(2018). |
入库方式： OAI收割
来源：植物研究所
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