The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes
文献类型：期刊论文
| 作者 | Li, Shan5; Xu, Huijinlan; Ju, Zheng; Cao, Dongyan; Zhu, Hongliang; Fu, Daqi; Grierson, Donald3,4; Qin, Guozheng2 ; Luo, Yunbo; Zhu, Benzhong
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| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 176期号:1页码:891-909 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.17.01449 |
| 文献子类 | Article |
| 英文摘要 | Fruit development and ripening is regulated by genetic and environmental factors and is of critical importance for seed dispersal, reproduction, and fruit quality. Tomato (Solanum lycopersicum) ripening inhibitor (rin) mutant fruit have a classic ripening-inhibited phenotype, which is attributed to a genomic DNA deletion resulting in the fusion of two truncated transcription factors, RIN and MC. In wild-type fruit, RIN, aMADS-box transcription factor, is a key regulator of the ripening gene expression network, with hundreds of gene targets controlling changes in color, flavor, texture, and taste during tomato fruit ripening; MC, on the other hand, has low expression in fruit, and the potential functions of the RIN-MC fusion gene in ripening remain unclear. Here, overexpression of RIN-MC in transgenic wild-type cv Ailsa Craig tomato fruits impaired several ripening processes, and down-regulating RIN-MC expression in the rin mutant was found to stimulate the normal yellow mutant fruit to produce a weak red color, suggesting a distinct negative role for RIN-MC in tomato fruit ripening. By comparative transcriptome analysis of rin and rin 35S:: RIN-MC RNA interference fruits, a total of 1,168 and 1,234 genes were identified as potential targets of RIN-MC activation and inhibition. Furthermore, the RIN-MC fusion gene was shown to be translated into a chimeric transcription factor that was localized to the nucleus and was capable of protein interactions with other MADS-box factors. These results indicated that tomato RIN-MC fusion plays a negative role in ripening and encodes a chimeric transcription factor that modulates the expression of many ripening genes, thereby contributing to the rin mutant phenotype. |
| 学科主题 | Plant Sciences |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | TOMATO FRUITFULL HOMOLOGS ; TRANSIENT EXPRESSION ; PROTEIN INTERACTIONS ; ETHYLENE ; DOMAIN ; IDENTIFICATION ; REGULATOR ; ARABIDOPSIS ; EVOLUTION ; GENETICS |
| 语种 | 英语 |
| WOS记录号 | WOS:000419675300068 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC 31571894, 31271959] ; Education Foundation of Da Bei Nong Group [1061-2415002] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20425]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Nottingham, Sch Biosci, Div Plant & Crop Sci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England 3.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China 4.Hubei Engn Univ, Hubei Prov Res Ctr Engn Technol Utilizat Bot Func, Xiaogan 432000, Peoples R China 5.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shan,Xu, Huijinlan,Ju, Zheng,et al. The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes[J]. PLANT PHYSIOLOGY,2018,176(1):891-909. |
| APA | Li, Shan.,Xu, Huijinlan.,Ju, Zheng.,Cao, Dongyan.,Zhu, Hongliang.,...&Zhu, Benzhong.(2018).The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes.PLANT PHYSIOLOGY,176(1),891-909. |
| MLA | Li, Shan,et al."The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes".PLANT PHYSIOLOGY 176.1(2018):891-909. |
入库方式： OAI收割
来源：植物研究所
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