Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance
文献类型：期刊论文
| 作者 | Zhang, Haiyan2; Yu, Pengli2; Zhao, Jiuhai3; Jiang, Hongling3; Wang, Haiyang4; Zhu, Yingfang5; Botella, Miguel A.; Samaj, Jozef7; Li, Chuanyou3; Lin, Jinxing2,8 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2018 |
| 卷号 | 217期号:2页码:799-812 |
| 关键词 | Arabidopsis long-distance movement mobile signal pathogen resistance prosystemin systemin tomato |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.14858 |
| 文献子类 | Article |
| 英文摘要 | Systemin (SYS), an octadecapeptide hormone processed from a 200-amino-acid precursor (prosystemin, PS), plays a central role in the systemic activation of defense genes in tomato in response to herbivore and pathogen attacks. However, whether PS mRNA is transferable and its role in systemic defense responses remain unknown. We created the transgenic tomato PS gene tagged with the green fluorescent protein (PS-GFP) using a shoot-or root-specific promoter, and the constitutive 35S promoter in Arabidopsis. Subcellular localization of PS-/SYS-GFP was observed using confocal laser scanning microscopy and gene transcripts were determined using quantitative real-time PCR. In Arabidopsis, PS protein can be processed and SYS is secreted. Shoot-/root-specific expression of PS-GFP in Arabidopsis, and grafting experiments, revealed that the PS mRNA moves in a bi-directional manner. We also found that ectopic expression of PS improves Arabidopsis resistance to the necrotrophic fungus Botrytis cinerea, consistent with substantial upregulation of the transcript levels of specific pathogen-responsive genes. Our results provide novel insights into the multifaceted mechanism of SYS signaling transport and its potential application in genetic engineering for increasing pathogen resistance across diverse plant families. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1469-8137 |
| WOS关键词 | LONG-DISTANCE TRAFFICKING ; PLANT DEFENSIVE GENES ; RECEPTOR KINASE ; BIOLOGICAL-ACTIVITY ; ELICITOR PEPTIDES ; BOTRYTIS-CINEREA ; TOBACCO PLANTS ; WOUND RESPONSE ; SIGNAL ; IMMUNITY |
| 语种 | 英语 |
| WOS记录号 | WOS:000419324000030 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470292, 31270313, 90817010] ; National Program of Sustainability I, MEYS, Czech Republic [LO1204] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20440]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Beijing Forestry Univ, Coll Biol Sci, Beijing 100083, Peoples R China 2.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China 5.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06411 USA 6.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA 7.Botella, Miguel A.] Univ Malaga, CSIC, Inst Hortofruticultura Subtrop & Mediterranea La, Dept Biol Mol & Bioquim,IHSM UMA, Campus Teatinos, E-29071 Malaga, Spain 8.Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Dept Cell Biol, Olomouc, Czech Republic |
| 推荐引用方式 GB/T 7714 | Zhang, Haiyan,Yu, Pengli,Zhao, Jiuhai,et al. Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance[J]. NEW PHYTOLOGIST,2018,217(2):799-812. |
| APA | Zhang, Haiyan.,Yu, Pengli.,Zhao, Jiuhai.,Jiang, Hongling.,Wang, Haiyang.,...&Lin, Jinxing.(2018).Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance.NEW PHYTOLOGIST,217(2),799-812. |
| MLA | Zhang, Haiyan,et al."Expression of tomato prosystemin gene in Arabidopsis reveals systemic translocation of its mRNA and confers necrotrophic fungal resistance".NEW PHYTOLOGIST 217.2(2018):799-812. |
入库方式： OAI收割
来源：植物研究所
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