Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds
文献类型：期刊论文
| 作者 | Yin, Dan-Dan; Li, Shan-Shan; Shu, Qing-Yan; Gu, Zhao-Yu; Wu, Qian; Feng, Cheng-Yong; Xu, Wen-Zhong; Wang, Liang-Sheng |
| 刊名 | GENE
 |
| 出版日期 | 2018 |
| 卷号 | 666页码:72-82 |
| ISSN号 | 0378-1119 |
| 关键词 | miRNA lncRNA alpha-Linolenic acid Target gene Tree peony seed Paeonia |
| DOI | 10.1016/j.gene.2018.05.011 |
| 文献子类 | Article |
| 英文摘要 | MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) act as important molecular regulators in a wide range of biological processes during plant development and seed formation, including oil production. Tree peony seeds contain > 90% unsaturated fatty acids (UFAs) and high proportions of a-linolenic acid (ALA, > 40%). To dissect the non-coding RNAs (ncRNAs) pathway involved in fatty acids synthesis in tree peony seeds, we construct six small RNA libraries and six transcriptome libraries from developing seeds of two cultivars (J and S) containing different content of fatty acid compositions. After deep sequencing the RNA libraries, the ncRNA expression profiles of tree peony seeds in two cultivars were systematically and comparatively analyzed. A total of 318 known and 153 new miRNAs and 22,430 lncRNAs were identified, among which 106 conserved and 9 novel miRNAs and 2785 lncRNAs were differentially expressed between the two cultivars. In addition, potential target genes of the microRNA and lncRNAs were also predicted and annotated. Among them, 9 miRNAs and 39 lncRNAs were predicted to target lipid related genes. Results showed that all of miR414, miR156b, miR2673b, miR7826, novel-m0027-5p, TR24651 vertical bar c0_gl, TR24544 vertical bar c0_g15, and TR27305 vertical bar c0_g1 were up-regulated and expressed at a higher level in high-ALA cultivar J when compared to low-ALA cultivar S, suggesting that these ncRNAs and target genes are possibly involved in different fatty acid synthesis and lipid metabolism through post-transcriptional regulation. These results provide a better understanding of the roles of ncRNAs during fatty acid biosynthesis and metabolism in tree peony seeds. |
| 学科主题 | Genetics & Heredity |
| 电子版国际标准刊号 | 1879-0038 |
| 出版地 | AMSTERDAM |
| WOS关键词 | ALPHA-LINOLENIC ACID ; GAS-CHROMATOGRAPHY ; BRASSICA-NAPUS ; OIL CONTENT ; GENE ; ARABIDOPSIS ; CULTIVARS ; STRESS ; MASS ; BIOGENESIS |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000445438700009 |
| 资助机构 | Beijing Agricultural Science and Technology Program [Z161100000916015] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20458]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Shu, Qing-Yan 3.Gu, Zhao-Yu 4.Wu, Qian 5.Li, Shan-Shan 6.[Yin, Dan-Dan 7.Feng, Cheng-Yong 8.Xu, Wen-Zhong 9.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China 10.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Dan-Dan,Li, Shan-Shan,Shu, Qing-Yan,et al. Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds[J]. GENE,2018,666:72-82. |
| APA | Yin, Dan-Dan.,Li, Shan-Shan.,Shu, Qing-Yan.,Gu, Zhao-Yu.,Wu, Qian.,...&Wang, Liang-Sheng.(2018).Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds.GENE,666,72-82. |
| MLA | Yin, Dan-Dan,et al."Identification of microRNAs and long non-coding RNAs involved in fatty acid biosynthesis in tree peony seeds".GENE 666(2018):72-82. |
入库方式： OAI收割
来源：植物研究所
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