Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii
文献类型：期刊论文
| 作者 | Li, Jing-Yi; Li, Dong; Du, Xue2; Li, Hui; Wang, Di; Xing, Quan; Yao, Ran1; Sun, Mei-Yu; Shi, Lei
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| 刊名 | ENVIRONMENTAL AND EXPERIMENTAL BOTANY
 |
| 出版日期 | 2018 |
| 卷号 | 156页码:298-315 |
| 关键词 | Drynaria roosii Naringin/neoeriocitrin synthesis Modular organization analysis UVC irradiation WGCNA |
| ISSN号 | 0098-8472 |
| DOI | 10.1016/j.envexpbot.2018.09.017 |
| 文献子类 | Article |
| 英文摘要 | Drynaria roosii is a traditional medicinal fern, whose rhizome is particularly valued for its effective flavonoid components of naringin/neoeriocitrin. UV irradiation can induce flavonoid accumulation in plants, however the molecular mechanism of UVC-mediated naringin/neoeriocitrin synthesis in D. roosii has never been reported. Our HPLC-tandem mass spectrometry (MS/MS) results showed that UVC irradiation increased naringin/neoeriocitrin contents (NNCs) based on irradiation dosage in D. roosii. According to the NGS data, DEG-based heat map analysis revealed up-regulation of naringin/neoeriocitrin synthetic genes was more obvious in 12h UVC irradiation (UV dose = 81.648 kJ m(-2)) when compared with 24h (UV dose = 163.296 kJ m(-2)), especially for PAL, C3'H and HCT in old rhizomes. Through systems biology method of modular organization analysis, we clustered 15,678 DEGs into 19 modules, and calculated correlation coefficients between modules and samples, as well as modules and NNCs. Four significant naringin-related modules were achieved and displayed high correlations with specific samples. Moreover, weighted gene co-expression network analysis results discovered that 4CL, CHS and HCT, C3H acted as the hub responsive genes to UVC irradiation involved in naringin and neoeriocitrin synthesis respectively, and presented high co-expression with MYB/bHLH-regulated DEGs. Overall, we demonstrated that UVC irradiation up-regulated the naringin/neoeriocitrin related gene expression in separate new and old rhizomes to enhance NNCs in a dose-dependent manner. Hormetic UVC dose ranges improving NNCs in D. roosii were established for the first time. Our work also provided new insights into the study of secondary metabolites in medicinal plants. |
| 学科主题 | Plant Sciences ; Environmental Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1873-7307 |
| WOS关键词 | FLAVONOID BIOSYNTHESIS ; ANTIOXIDANT CAPACITY ; TRANSCRIPTION FACTOR ; ARABIDOPSIS ; NETWORK ; LIGHT ; IDENTIFICATION ; ACCUMULATION ; METABOLOME ; INDUCTION |
| 语种 | 英语 |
| WOS记录号 | WOS:000448093600029 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81573519, 31600264] ; Key Projects of the Chinese Academy of Sciences [KZCC-EW-103-3] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20574]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Semicond, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jing-Yi,Li, Dong,Du, Xue,et al. Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2018,156:298-315. |
| APA | Li, Jing-Yi.,Li, Dong.,Du, Xue.,Li, Hui.,Wang, Di.,...&Shi, Lei.(2018).Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,156,298-315. |
| MLA | Li, Jing-Yi,et al."Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 156(2018):298-315. |
入库方式： OAI收割
来源：植物研究所
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