Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation
文献类型：期刊论文
| 作者 | Yin, Dan-Dan; Xu, Wen-Zhong; Shu, Qing-Yan; Li, Shan-Shan; Wu, Qian; Feng, Cheng-Yong; Gu, Zhao-Yu; Wang, Liang-Sheng |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2018 |
| 卷号 | 274页码:212-222 |
| ISSN号 | 0168-9452 |
| 关键词 | alpha-Linolenic acid Comparative transcriptome Fatty acid desaturase Paeoniaceae Tree peony |
| DOI | 10.1016/j.plantsci.2018.05.027 |
| 文献子类 | Article |
| 英文摘要 | alpha-linolenic acid (ALA) deficiency and a skewed m6: co3 fatty acid ratio in the diet are thought to be a major cause for the high incidence of cardiovascular, inflammatory, and autoimmune diseases. Recent years, tree peony (Paeonia suffruticosa Andr.) with the high proportion of ALA (more than 45% in seed oil) is widely concerned. However, the underlying accumulation mechanism of the ALA in tree peony seeds remains unknown. In this study, comparative transcriptome analysis was performed between two cultivars ('Saiguifer and 'Jingshenhuanfa') with different ALA contents. The analysis of the metabolic enzymes associated with ALA biosynthesis and temporal accumulation patterns of unsaturated fatty acids demonstrated the importance of microsomal omega-3 fatty acid desaturase 3 (FAD3). Moreover, PsFAD3 gene was identified from tree peony seeds, which was located in endoplasmic reticulum and the expression levels of PsFAD3 were consistent with ALA accumulation patterns in seeds. Heterologous expression in Saccharomyces cerevisiae and Arabidopsis thaliana confirmed that the isolated PsFAD3 protein could catalyze ALA synthesis. These results indicated that PsFAD3 was involved in the synthesis of ALA in seeds and could be exploited by the genetic breeding of new cultivars with high ALA content in tree peony as well as other potential crops. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | CLARE |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; MOLECULAR-CLONING ; RNA-SEQ ; GENES ; EXPRESSION ; IDENTIFICATION ; ARABIDOPSIS ; SEED ; TRANSCRIPTOME ; BIOSYNTHESIS |
| 语种 | 英语 |
| 出版者 | ELSEVIER IRELAND LTD |
| WOS记录号 | WOS:000442068200024 |
| 资助机构 | Beijing Agricultural Science and Technology Program [Z161100000916015] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20627]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Feng, Cheng-Yong 2.Wu, Qian 3.Li, Shan-Shan 4.Shu, Qing-Yan 5.Xu, Wen-Zhong 6.[Yin, Dan-Dan 7.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 8.Gu, Zhao-Yu 9.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 10.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Dan-Dan,Xu, Wen-Zhong,Shu, Qing-Yan,et al. Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation[J]. PLANT SCIENCE,2018,274:212-222. |
| APA | Yin, Dan-Dan.,Xu, Wen-Zhong.,Shu, Qing-Yan.,Li, Shan-Shan.,Wu, Qian.,...&Wang, Liang-Sheng.(2018).Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation.PLANT SCIENCE,274,212-222. |
| MLA | Yin, Dan-Dan,et al."Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high alpha-linolenic acid accumulation".PLANT SCIENCE 274(2018):212-222. |
入库方式： OAI收割
来源：植物研究所
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