In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy
文献类型：期刊论文
| 作者 | Man, Yi; Zhao, Yuanyuan; Ye, Rong; Lin, Jinxing1; Jing, Yanping |
| 刊名 | JOURNAL OF PLANT PHYSIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 220页码:136-144 |
| 关键词 | Casparian strip Lignin Suberin Label-free analysis Stimulated Raman scattering microscopy Maize |
| ISSN号 | 0176-1617 |
| DOI | 10.1016/j.jplph.2017.11.002 |
| 文献子类 | Article |
| 英文摘要 | The Casparian strip, a barrier to the apoplastic movement of solutes from the cortex to the stele, is essential for the exclusion of salts, selective nutrient uptake, and many other processes. To date, extensive studies have focused on the physiological functions of endodermal Casparian strips. However, the chemical deposition nature of Casparian strips, as well as its relevance with respect to diffusion barrier functions, remains to be further elucidated. Here, we revealed three developmental stages of Casparian strips in maize primary roots using a traditional fluorescent staining method. Apoplastic permeability tests demonstrated that the barrier function of Casparian strips is largely related to their developmental stage and the pattern of lignin and suberin deposits. Fourier transform infrared (FTIR) analysis showed that the Casparian strips from the roots exhibited significant absorption bands characteristic of lignin and suberin, implying that the Casparian strips in maize primary roots consist largely of lignin and suberin. Furthermore, we developed a new method for label-free, in vivo structural, and biochemical analysis of Casparian strips based on stimulated Raman scattering (SRS) microscopy. Using SRS microscopy, we found that lignin and suberin accumulate simultaneously during the Casparian strip formation process. Based on these results, we propose a potential application of SRS for the chemical composition analysis of plant Casparian strips in situ. |
| 学科主题 | Plant Sciences |
| 出版地 | MUNICH |
| 电子版国际标准刊号 | 1618-1328 |
| WOS关键词 | LABEL-FREE ; FINE-STRUCTURE ; ROOTS ; LIGNIN ; GROWTH ; TRANSPORT ; BARRIERS ; SUBERIN ; MAIZE ; SALT |
| 语种 | 英语 |
| WOS记录号 | WOS:000422925800014 |
| 出版者 | ELSEVIER GMBH |
| 资助机构 | National Key Research and Development Program of China [2017YFD0600201] ; Program of Introducing Talents of Discipline to Universities (111 project) [B13007] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/20679]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Box 162,35 Qinghua East Rd, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Man, Yi,Zhao, Yuanyuan,Ye, Rong,et al. In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy[J]. JOURNAL OF PLANT PHYSIOLOGY,2018,220:136-144. |
| APA | Man, Yi,Zhao, Yuanyuan,Ye, Rong,Lin, Jinxing,&Jing, Yanping.(2018).In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy.JOURNAL OF PLANT PHYSIOLOGY,220,136-144. |
| MLA | Man, Yi,et al."In vivo cytological and chemical analysis of Casparian strips using stimulated Raman scattering microscopy".JOURNAL OF PLANT PHYSIOLOGY 220(2018):136-144. |
入库方式： OAI收割
来源：植物研究所
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