SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening
文献类型：期刊论文
| 作者 | Ji, Dongchao1; Cui, Xiaomin1; Qin, Guozheng ; Chen, Tong; Tian, Shiping1,2
|
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 184期号:4页码:2168-2181 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.20.01203 |
| 文献子类 | Article |
| 英文摘要 | The tomato membrane protein SlFERL regulates fruit ripening via modulating ethylene production. Fruit ripening is a complex and genetically programmed process modulated by transcription factors, hormones, and other regulators. However, the mechanism underlying the regulatory loop involving the membrane-protein targets of RIPENING-INHIBITOR (RIN) remains poorly understood. To unravel the function of tomato (Solanum lycopersicum) FERONIA Like (SlFERL), a putative MADS-box transcription factor target gene, we investigated and addressed the significance of SlFERL in fruit ripening by combining reverse genetics, biochemical, and cytological analyses. Here, we report that RIN and Tomato AGAMOUS-LIKE1 (TAGL1) directly bind to the promoter region of SlFERL and further activate its expression transcriptionally, suggesting a potential role of SlFERL in fruit ripening. Overexpression of SlFERL significantly accelerated the ripening process of tomato fruit, whereas RNA interference knockdown of SlFERL resulted in delayed fruit ripening. Moreover, a surface plasmon resonance assay coupled with tandem mass spectrometry and a protein interaction assay revealed that SlFERL interacts with the key enzyme S-adenosyl-Met synthetase 1 (SlSAMS1) in the ethylene biosynthesis pathway, leading to increased S-adenosyl-Met accumulation and elevated ethylene production. Thus, SlFERL serves as a positive regulator of ethylene production and fruit ripening. This study provides clues to the molecular regulatory networks underlying fruit ripening. |
| 学科主题 | Plant Sciences |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | ADENOSYL-L-METHIONINE ; ETHYLENE BIOSYNTHESIS ; RECEPTOR KINASES ; INHIBITOR RIN ; TOMATO ; FERONIA ; ARABIDOPSIS ; GENE ; REVEAL ; IDENTIFICATION |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000601303200046 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31925035, 32072637] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21490]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Key Lab Post Harvest Handling Fruits, Minist Agr, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Dongchao,Cui, Xiaomin,Qin, Guozheng,et al. SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening[J]. PLANT PHYSIOLOGY,2020,184(4):2168-2181. |
| APA | Ji, Dongchao,Cui, Xiaomin,Qin, Guozheng,Chen, Tong,&Tian, Shiping.(2020).SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening.PLANT PHYSIOLOGY,184(4),2168-2181. |
| MLA | Ji, Dongchao,et al."SlFERL Interacts with S-Adenosylmethionine Synthetase to Regulate Fruit Ripening".PLANT PHYSIOLOGY 184.4(2020):2168-2181. |
入库方式： OAI收割
来源：植物研究所
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