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Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato

文献类型：期刊论文

作者Wang, Peiwen2; Wang, Yuying; Wang, Weihao; Chen, Tong; Tian, Shiping2; Qin, Guozheng2
刊名COMMUNICATIONS BIOLOGY
出版日期2020
卷号3期号:1
DOI10.1038/s42003-020-01474-3
文献子类Article
英文摘要Carotenoids are natural pigments that are indispensable to plants and humans, whereas the regulation of carotenoid biosynthesis by post-translational modification remains elusive. Here, we show that a tomato E3 ubiquitin ligase, Plastid Protein Sensing RING E3 ligase 1 (PPSR1), is responsible for the regulation of carotenoid biosynthesis. PPSR1 exhibits self-ubiquitination activity and loss of PPSR1 function leads to an increase in carotenoids in tomato fruit. PPSR1 affects the abundance of 288 proteins, including phytoene synthase 1 (PSY1), the key rate-limiting enzyme in the carotenoid biosynthetic pathway. PSY1 contains two ubiquitinated lysine residues (Lys380 and Lys406) as revealed by the global analysis and characterization of protein ubiquitination. We provide evidence that PPSR1 interacts with PSY1 precursor protein and mediates its degradation via ubiquitination, thereby affecting the steady-state level of PSY1 protein. Our findings not only uncover a regulatory mechanism for controlling carotenoid biosynthesis, but also provide a strategy for developing carotenoid-enriched horticultural crops. Wang et al. report on the role of a novel E3 ubiquitin ligase, Plastid Protein Sensing RING E3 ligase 1 (PPSR1), during tomato fruit ripening and find that it interacts with phytoene synthase 1 (PSY1) precursor protein and mediates its degradation via ubiquitination. This affects the steady-state level of PSY1 protein, the key rate-limiting enzyme in the carotenoid biosynthetic pathway. This study may provide a strategy for developing carotenoid-enriched horticultural crops.
学科主题Biology ; Multidisciplinary Sciences
出版地BERLIN
电子版国际标准刊号2399-3642
WOS关键词E3 LIGASE ATCHIP ; TRANSCRIPTION FACTOR ; PROTEASOME SYSTEM ; GENE-EXPRESSION ; FRUITFULL HOMOLOGS ; PROTEIN ; DEGRADATION ; ARABIDOPSIS ; ACCUMULATION ; IDENTIFICATION
WOS研究方向Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000598831000002
出版者NATURE RESEARCH
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31925035, 31930086, 31572174]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21493]  
专题中科院北方资源植物重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Peiwen,Wang, Yuying,Wang, Weihao,et al. Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato[J]. COMMUNICATIONS BIOLOGY,2020,3(1).
APA Wang, Peiwen,Wang, Yuying,Wang, Weihao,Chen, Tong,Tian, Shiping,&Qin, Guozheng.(2020).Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato.COMMUNICATIONS BIOLOGY,3(1).
MLA Wang, Peiwen,et al."Ubiquitination of phytoene synthase 1 precursor modulates carotenoid biosynthesis in tomato".COMMUNICATIONS BIOLOGY 3.1(2020).

入库方式： OAI收割

来源：植物研究所

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