Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3
文献类型：期刊论文
| 作者 | Xu, Meilong1,2; Tong, Qian1; Wang, Yi1; Wang, Zemin1; Xu, Guangzhao1; Elias, Gathunga Kirabi1; Li, Shaohua1 ; Liang, Zhenchang3
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| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 61期号:4页码:775-786 |
| 关键词 | Cold and osmotic stresses Grapevine LEA genes Overexpression of VamDHN3 gene Phylogenetic analysis Transcriptomic analysis |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcaa004 |
| 文献子类 | Article |
| 英文摘要 | Late embryogenesis abundant (LEA) proteins comprise a large family that plays important roles in the regulation of abiotic stress, however, no in-depth analysis of LEA genes has been performed in grapevine to date. In this study, we analyzed a total of 52 putative LEA genes in grapevine at the genomic and transcriptomic level, compiled expression profiles of four selected (V. amurensis) VamLEA genes under cold and osmotic stresses, and studied the potential function of the V. amurensis DEHYDRIN3 (VamDHN3) gene in grapevine callus. The 52 LEA proteins were classified into seven phylogenetic groups. RNA-seq and quantitative real-time PCR results demonstrated that a total of 16 and 23 VamLEA genes were upregulated under cold and osmotic stresses, respectively. In addition, overexpression of VamDHN3 enhanced the stability of the cell membrane in grapevine callus, suggesting that VamDHN3 is involved in osmotic regulation. These results provide fundamental knowledge for the further analysis of the biological roles of grapevine LEA genes in adaption to abiotic stress. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1471-9053 |
| WOS关键词 | LATE-EMBRYOGENESIS-ABUNDANT ; HABROCHAITES ENHANCES TOLERANCE ; GENOME-WIDE IDENTIFICATION ; DEHYDRIN GENE ; EXPRESSION ANALYSIS ; ESCHERICHIA-COLI ; OXIDATIVE STRESS ; ABIOTIC STRESSES ; PROTEINS ; OVEREXPRESSION |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000553476300011 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Grape breeding project of Ningxia, China [NXNYYZ201502] ; major science and technology program of Ningxia Hui Autonomous region, China [2016BZ06] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21653]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Beijing Key Lab Grape Sci & Enol,CAS Key Lab Plan, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.State Key Lab Seedling Bioengn, Yinchuan 750004, Ningxia, Peoples R China 4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Meilong,Tong, Qian,Wang, Yi,et al. Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3[J]. PLANT AND CELL PHYSIOLOGY,2020,61(4):775-786. |
| APA | Xu, Meilong.,Tong, Qian.,Wang, Yi.,Wang, Zemin.,Xu, Guangzhao.,...&Liang, Zhenchang.(2020).Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3.PLANT AND CELL PHYSIOLOGY,61(4),775-786. |
| MLA | Xu, Meilong,et al."Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3".PLANT AND CELL PHYSIOLOGY 61.4(2020):775-786. |
入库方式： OAI收割
来源：植物研究所
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