SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)
文献类型：期刊论文
| 作者 | Cai, Jianghua1; Chen, Tong; Wang, Ying1; Qin, Guozheng1 ; Tian, Shiping1
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| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 183期号:2页码:717-732 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.20.00120 |
| 文献子类 | Article |
| 英文摘要 | Programmed cell death (PCD), a highly regulated feature of the plant immune response, involves multiple molecular players. Remorins (REMs) are plant-specific proteins with varied biological functions, but their function in PCD and plant defense remains largely unknown. Here, we report a role for remorin in disease resistance, immune response, and PCD regulation. Overexpression of tomato (Solanum lycopersicum)REMORIN1(SlREM1) increased susceptibility of tomato to the necrotrophic fungusBotrytis cinereaand heterologous expression of this gene triggered cell death inNicotiana benthamianaleaves. Further investigation indicated that amino acids 173 to 187 and phosphorylation of SlREM1 played key roles in SlREM1-triggered cell death. Intriguingly, multiple tomato REMs induced cell death inN.benthamianaleaves. Yeast two-hybrid, split luciferase complementation, and coimmunoprecipitation assays all demonstrated that remorin proteins could form homo- and heterocomplexes. Using isobaric tags for relative and absolute quantitative proteomics, we identified that some proteins related to cell death regulation, as well asN.benthamianaRESPIRATORY BURST OXIDASE HOMOLOG B (which is essential for reactive oxygen species production), were notably upregulated inSlREM1-expressing leaves. Heterologous expression ofSlREM1increased reactive oxygen species accumulation and triggered other cell death regulators. Our findings indicate that SlREM1 is a positive regulator of plant cell death and provide clues for understanding the PCD molecular regulatory network in plants. A tomato remorin protein causes cell death by increasing the accumulation of reactive oxygen species and other defense factors. |
| 学科主题 | Plant Sciences |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | PLASMA-MEMBRANE LOCALIZATION ; E3 UBIQUITIN LIGASE ; TRANSCRIPTION FACTOR ; HYPERSENSITIVE RESPONSE ; PROTEIN-PHOSPHORYLATION ; POSITIVE REGULATOR ; POLLEN DEVELOPMENT ; FRUIT SENESCENCE ; DEFENSE RESPONSE ; BOTRYTIS-CINEREA |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000541865100037 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31530057, 31672210] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21710]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Jianghua,Chen, Tong,Wang, Ying,et al. SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)[J]. PLANT PHYSIOLOGY,2020,183(2):717-732. |
| APA | Cai, Jianghua,Chen, Tong,Wang, Ying,Qin, Guozheng,&Tian, Shiping.(2020).SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1).PLANT PHYSIOLOGY,183(2),717-732. |
| MLA | Cai, Jianghua,et al."SlREM1 Triggers Cell Death by Activating an Oxidative Burst and Other Regulators(1)".PLANT PHYSIOLOGY 183.2(2020):717-732. |
入库方式： OAI收割
来源：植物研究所
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