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Gene body demethylation increases expression and is associated with self-pruning during grape genome duplication

文献类型：期刊论文

作者Zou, Luming1,5; Liu, Wenwen1,5; Zhang, Zhan1,5,6; Edwards, Everard J.; Gathunga, Elias Kirabi1,5; Fan, Peige5; Duan, Wei5; Li, Shaohua1,5; Liang, Zhenchang2,5
刊名HORTICULTURE RESEARCH
出版日期2020
卷号7期号:1
ISSN号2662-6810
DOI10.1038/s41438-020-0303-7
文献子类Article
英文摘要A colchicine-induced autotetraploid grapevine exhibiting potentially valuable agronomic traits for grape production and breeding, including self-pruning, was identified. This study investigated DNA methylation variation and its role in gene expression during self-pruning in the autotetraploid grapevine. We used RNA-Seq to estimate differentially expressed genes between diploid and autotetraploid grapevine shoot tips. The genes showing increases in the autotetraploid were mainly related to stress response pathways, whereas those showing decreases in the autotetraploid were related to biological metabolism and biosynthesis. Whole-genome bisulfite sequencing was performed to produce single-base methylomes for the diploid and autotetraploid grapevines. Comparison between the methylomes revealed that they were conserved in CG and CHG contexts. In the autotetraploid grapevine, hypodifferentially methylated regions (DMRs) and hyper-DMRs in the gene body increased or decreased gene expression, respectively. Our results indicated that a hypo-DMR in the ACO1 gene body increased its expression and might promote self-pruning. This study reports that hypo-DMRs in the gene body increase gene expression in plants and reveals the mechanism underlying the changes in the modifications affecting gene expression during genome duplication. Overall, our results provide valuable information for understanding the relationships between DNA methylation, gene expression, and autotetraploid breeding in grape.
学科主题Plant Sciences ; Genetics & Heredity ; Horticulture
出版地NANJING
电子版国际标准刊号2052-7276
WOS关键词DNA METHYLATION ; ETHYLENE ; POLYPLOIDY ; ABSCISSION ; ABA ; AUXIN ; BIOSYNTHESIS ; RESISTANCE ; INDUCTION ; RETENTION
WOS研究方向Plant Sciences ; Genetics & Heredity ; Agriculture
语种英语
WOS记录号WOS:000538044000006
出版者NANJING AGRICULTURAL UNIV
资助机构National Key Research and Development Program of China [2018YFD1000105] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772266] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203] ; STS Project of the Chinese Academy of Sciences [KFJ-STS-ZDTP-025]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/21731]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Edwards, Everard J.] CSIRO Agr & Food, Locked Bag 2, Glen Osmond, SA 5064, Australia
3.Shaanxi Normal Univ, Coll Life Sci, Xian 041004, Shanxi, Peoples R China
4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China
5.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zou, Luming,Liu, Wenwen,Zhang, Zhan,et al. Gene body demethylation increases expression and is associated with self-pruning during grape genome duplication[J]. HORTICULTURE RESEARCH,2020,7(1).
APA Zou, Luming.,Liu, Wenwen.,Zhang, Zhan.,Edwards, Everard J..,Gathunga, Elias Kirabi.,...&Liang, Zhenchang.(2020).Gene body demethylation increases expression and is associated with self-pruning during grape genome duplication.HORTICULTURE RESEARCH,7(1).
MLA Zou, Luming,et al."Gene body demethylation increases expression and is associated with self-pruning during grape genome duplication".HORTICULTURE RESEARCH 7.1(2020).

入库方式： OAI收割

来源：植物研究所

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