Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus
文献类型：期刊论文
| 作者 | Liu, Dantong1; Ji, Hongying1; Yang, Zhenle |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 61期号:1页码:118-129 |
| ISSN号 | 0032-0781 |
| 关键词 | Cyperus esculentus Tuber Diacylglycerol acyltransferase Oil Triacylglycerol Fatty acid |
| DOI | 10.1093/pcp/pcz184 |
| 文献子类 | Article |
| 英文摘要 | Cyperus esculentus is probably the only plant that is known to accumulate large amounts of oil in its tubers. However, the underlying metabolic mechanism and regulatory factors involved in oil synthesis of tubers are still largely unclear. In this study, one gene encoding type I diacylglycerol acyltransferase (DGAT) (CeDGAT1) and two genes encoding type II DGAT (CeDGAT2a and CeDGAT2b) from C esculentus were identified and functionally analyzed. All three DGAT genes were found to be expressed in tuber, root and leaf tissues. CeDGAT1 is highly expressed in roots and leaves, whereas CeDGAT2b is dominantly expressed in tubers. Furthermore, the temporal expression pattern of CeDGAT2b is well coordinated with the oil accumulation in developing tubers. When each CeDGAT was heterologously expressed in triacylglycerol (TAG)-deficient mutant of Saccharomyces cerevisiae, Arabidopsis thaliana wild type or its TAG1 mutant with AtDGAT1 disruption, only CeDGAT2b showed the ability to restore TAG biosynthesis with lipid body formation in yeast mutant, enhance seed oil production of Arabidopsis wild type and rescue multiple seed phenotypes of TAG1 mutant. In addition, CeDGAT2b was shown to have a substrate preference for unsaturated fatty acids toward TAG synthesis. Taken together, our results indicated that CeDGAT2b from C. esculentus is an actively functional protein and is most likely the major contributor to tuber oil biosynthesis containing common fatty acids, in contrast to oil-rich seeds and fruits where DGAT1 plays a more central role than DGAT2 in oil production accumulating normal fatty acids, whereas DGAT2 is a primary regulator for oil synthesis rich in unusual fatty acids. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 电子版国际标准刊号 | 1471-9053 |
| 出版地 | OXFORD |
| WOS关键词 | FATTY-ACID PROFILE ; SEED OIL ; TRIACYLGLYCEROL BIOSYNTHESIS ; ARABIDOPSIS ; EXTRACTION ; EXPRESSION ; NUT ; TRANSFORMATION ; TOCOPHEROL ; AVOCADO |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000522854500011 |
| 资助机构 | Natural Science Foundation of Beijing MunicipalityBeijing Natural Science Foundation [5151001] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371692] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21836]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Dantong,Ji, Hongying,Yang, Zhenle. Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus[J]. PLANT AND CELL PHYSIOLOGY,2020,61(1):118-129. |
| APA | Liu, Dantong,Ji, Hongying,&Yang, Zhenle.(2020).Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus.PLANT AND CELL PHYSIOLOGY,61(1),118-129. |
| MLA | Liu, Dantong,et al."Functional Characterization of Three Novel Genes Encoding Diacylglycerol Acyltransferase (DGAT} from Oil-Rich Tubers of Cyperus esculentus".PLANT AND CELL PHYSIOLOGY 61.1(2020):118-129. |
入库方式： OAI收割
来源：植物研究所
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