Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins
文献类型：期刊论文
| 作者 | Li, Hua2; Zhang, Zhanquan1; Qin, Guozheng1 ; He, Chang2; Li, Boqiang1; Tian, Shiping1,2
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| 刊名 | MSYSTEMS
 |
| 出版日期 | 2020 |
| 卷号 | 5期号:1 |
| 关键词 | actin Botrytis cinerea pathogenesis secretome |
| ISSN号 | 2379-5077 |
| DOI | 10.1128/mSystems.00732-19 |
| 文献子类 | Article |
| 英文摘要 | Actin is a vital component of the cytoskeleton of living cells and is involved in several complex processes. However, its functions in plant-pathogenic fungi are largely unknown. In this paper, we found that deletion of the Botrytis cinerea actin gene bcactA reduced growth and sporulation of B. cinerea and lowered virulence. Based on iTRAQ (isobaric tags for relative and absolute quantification)based proteomic analysis, we compared changes of the secretome in Delta bcactA and wild-type strains. A total of 40 proteins exhibited significant differences in abundance in Delta bcactA mutants compared with the wild type. These proteins included 11 down-accumulated cell wall-degrading enzymes (CWDEs). Among them, two CWDEs, cellobiohydrolase (BcCBH) and beta-endoglucanase (BcEG), were found to contribute to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating the secretion of extracellular virulence factors. These findings unveil previously unknown functions of BcactA to mediate the virulence of B. cinerea and provide new mechanistic insights into the role of BcactA in the complex pathogenesis of B. cinerea. IMPORTANCE The cytoskeleton is an important network that exists in cells of all domains of life. In eukaryotic cells, actin is a vital component of the cytoskeleton. Here, we report that BcactA, an actin protein in B. cinerea, can affect the growth, sporulation, and virulence of B. cinerea. Furthermore, iTRAQ-based proteomic analysis showed that BcactA affects the abundance of 40 extracellular proteins, including 11 down-accumulated CWDEs. Among them, two CWDEs, cellobiohydrolase (BcCBH) and beta-endoglucanase (BcEG), contributed to the virulence of B. cinerea, indicating that bcactA plays a crucial role in regulating extracellular virulence factors. These findings unveil previously unknown functions of BcactA in mediating growth, sporulation, and virulence of B. cinerea. |
| 学科主题 | Microbiology |
| 出版地 | WASHINGTON |
| WOS关键词 | PROTEOMIC ANALYSIS ; NADPH-OXIDASE ; GROWTH ; CYTOSKELETON ; ISOVARIANTS ; MECHANISMS ; REVEALS ; ENZYMES ; FUNGUS |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000518855000024 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 资助机构 | Key Program of National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930086, 31530057] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association CAS [2018107] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21868]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 3.Minist Agr China, Key Lab Postharvest Handling Fruits, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hua,Zhang, Zhanquan,Qin, Guozheng,et al. Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins[J]. MSYSTEMS,2020,5(1). |
| APA | Li, Hua,Zhang, Zhanquan,Qin, Guozheng,He, Chang,Li, Boqiang,&Tian, Shiping.(2020).Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins.MSYSTEMS,5(1). |
| MLA | Li, Hua,et al."Actin Is Required for Cellular Development and Virulence of Botrytis cinerea via the Mediation of Secretory Proteins".MSYSTEMS 5.1(2020). |
入库方式： OAI收割
来源：植物研究所
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