Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis)
文献类型：期刊论文
| 作者 | Zhang, Qingfen1; Qi, Dongmei; Dong, Xiaobing; Li, Xiaoxia; Cheng, Liqin; Liu, Hui; Chen, Shuangyan; Rajora, Om P.; Li, Xiu-Qing; Liu, Gongshe
|
| 刊名 | BOTANY
 |
| 出版日期 | 2020 |
| 卷号 | 98期号:3页码:137-146 |
| 关键词 | sheepgrass (Leymus chinensis) amino acid composition protein content nitrogen-to-protein conversion factor |
| ISSN号 | 1916-2790 |
| DOI | 10.1139/cjb-2019-0082 |
| 文献子类 | Article |
| 英文摘要 | The protein content of plants is commonly estimated by multiplying the total nitrogen content (Kjeldahl; KN) by a nitrogen-to-protein conversion factor of 6.25. This method is based on the incorrect assumption that all nitrogen in the ammonia/ammonium and organic substances in plants is protein nitrogen, usually resulting in overestimation of protein content. We have examined amino acid composition, amino acid nitrogen, total nitrogen (KN), and actual protein content (AP) determined from amino acid residues in 16 accessions of perennial sheepgrass (Leymus chinensis (Trin.) Tzvelev). We determined a new nitrogen-to-protein conversion factor, k(p), as the ratio of AP to KN, and applied this factor to estimate the total protein content (TP) as KN x k(p). The non-protein nitrogen accounted for 40.5% to 62.4% of the total nitrogen. The average k(p) value was 3.17 overall, 3.20 in the accessions sampled at the jointing stage, and 3.15 in the accessions sampled at the flowering stage. The TP, calculated as KN x 3.17, was about half that of crude protein contents, calculated as KN x 6.25. Our study suggests that the AP-based k(p) of 3.17 can be used to more accurately estimate the total protein content in sheepgrass. |
| 学科主题 | Plant Sciences |
| 出版地 | OTTAWA |
| 电子版国际标准刊号 | 1916-2804 |
| WOS关键词 | COMMON ; PROLINE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000518797200002 |
| 出版者 | CANADIAN SCIENCE PUBLISHING |
| 资助机构 | National Key R&D Program of China [2018YFD1001000, 2016YFC0500700] ; Science and Technology Major Project of Inner Mongolia Autonomous Region of China ; Kulun Banner Science and Technology Poverty Alleviation Project of Chinese Academy of Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21869]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Rajora, Om P.] Univ New Brunswick, Fac Forestry & Environm Management, 28 Dineen Dr, Fredericton, NB E3B 5A3, Canada 4.Agr & Agri Food Canada, Fredericton Res & Dev Ctr, Fredericton, NB E3B 4Z7, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Qingfen,Qi, Dongmei,Dong, Xiaobing,et al. Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis)[J]. BOTANY,2020,98(3):137-146. |
| APA | Zhang, Qingfen.,Qi, Dongmei.,Dong, Xiaobing.,Li, Xiaoxia.,Cheng, Liqin.,...&Liu, Gongshe.(2020).Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis).BOTANY,98(3),137-146. |
| MLA | Zhang, Qingfen,et al."Amino acid composition, protein content and accurate nitrogen-to-protein conversion factor for sheepgrass (Leymus chinensis)".BOTANY 98.3(2020):137-146. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。