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Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

文献类型：期刊论文

作者Jiang, Jianfu; Liu, Xinna1; Liu, Chonghuai; Liu, Guotian1; Li, Shaohua1; Wang, Lijun1
刊名PLANT PHYSIOLOGY
出版日期2017
卷号173期号:2页码:1502-1518
ISSN号0032-0889
DOI10.3389/fpls.2017.01779
文献子类Article
英文摘要Heat stress is one of the primary abiotic stresses that limit crop production. Grape (Vitis vinifera) is a cultivated fruit with high economic value throughout the world, with its growth and development often influenced by high temperature. Alternative splicing (AS) is a widespread phenomenon increasing transcriptome and proteome diversity. We conducted high-temperature treatments (35 degrees C, 40 degrees C, and 45 degrees C) on grapevines and assessed transcriptomic (especially AS) and proteomic changes in leaves. We found that nearly 70% of the genes were alternatively spliced under high temperature. Intron retention (IR), exon skipping, and alternative donor/acceptor sites were markedly induced under different high temperatures. Among all differential AS events, IR was the most abundant up-and down-regulated event. Moreover, the occurrence frequency of IR events at 40 degrees C and 45 degrees C was far higher than at 35 degrees C. These results indicated that AS, especially IR, is an important posttranscriptional regulatory event during grape leaf responses to high temperature. Proteomic analysis showed that protein levels of the RNA-binding proteins SR45, SR30, and SR34 and the nuclear ribonucleic protein U1A gradually rose as ambient temperature increased, which revealed a reason why AS events occurred more frequently under high temperature. After integrating transcriptomic and proteomic data, we found that heat shock proteins and some important transcription factors such as MULTIPROTEIN BRIDGING FACTOR1c and HEAT SHOCK TRANSCRIPTION FACTOR A2 were involved mainly in heat tolerance in grape through up-regulating transcriptional (especially modulated by AS) and translational levels. To our knowledge, these results provide the first evidence for grape leaf responses to high temperature at simultaneous transcriptional, posttranscriptional, and translational levels.
学科主题Plant Sciences
出版地ROCKVILLE
电子版国际标准刊号1532-2548
WOS关键词HEAT-SHOCK PROTEINS ; STRESS RESPONSE ; SALICYLIC-ACID ; TRANSCRIPTION FACTOR ; EXPRESSION PROFILES ; PROTEOMIC ANALYSIS ; ARABIDOPSIS ; TOLERANCE ; ACCLIMATION ; THERMOTOLERANCE
语种英语
WOS记录号WOS:000414388400001
出版者AMER SOC PLANT BIOLOGISTS
资助机构Agricultural Science and Technology Innovation Program [CAAS-ASTIP-2016-ZFRI] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270718]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/22029]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou 450009, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Jianfu,Liu, Xinna,Liu, Chonghuai,et al. Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature[J]. PLANT PHYSIOLOGY,2017,173(2):1502-1518.
APA Jiang, Jianfu,Liu, Xinna,Liu, Chonghuai,Liu, Guotian,Li, Shaohua,&Wang, Lijun.(2017).Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature.PLANT PHYSIOLOGY,173(2),1502-1518.
MLA Jiang, Jianfu,et al."Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature".PLANT PHYSIOLOGY 173.2(2017):1502-1518.

入库方式： OAI收割

来源：植物研究所

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