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Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis

文献类型：期刊论文

作者Hao, Qing4; Ren, Hongxu; Zhu, Jin3; Wang, Liangsheng; Huang, Shouchen4; Liu, Zheng'an; Gao, Zhimin1; Shu, Qingyan
刊名PLANT CELL REPORTS
出版日期2017
卷号36期号:1页码:151-162
关键词SKP1 Paeonia suffruticosa PSK1 Salinity tolerance Flowering
ISSN号0721-7714
DOI10.3389/fpls.2017.01047
文献子类Article
英文摘要Our study is the first to demonstrate that PSK1 , a SKP1 -like gene homologue, is involved in salinity tolerance. Our functional characterization of PSK1 provides new insights into tree peony development. A homologous gene of S-phase kinase-associated protein1 (SKP1) was cloned from tree peony (Paeonia suffruticosa) and denoted as PSK1. The 462-bp open reading frame of PSK1 was predicted to encode a protein comprising 153 amino acids, with a molecular mass of 17 kDa. The full-length gene was 1,634 bp long and included a large 904-bp intron. PSK1 transcription was detected in all tissues, with the highest level observed in sepals, followed by leaves. Under salinity stress, overexpression of PSK1 in Arabidopsis resulted in increased germination percentages, cotyledon greening, and fresh weights relative to wild-type plants. Furthermore, transgenic Arabidopsis lines containing 35S::PSK1 displayed increased expression of genes that would be essential for reproduction and growth under salinity stress: ASK1, LEAFY, FT, and CO involved in flower development and flowering time as well as P5CS, RAB18, DREB, and SOD1-3 contributing to salinity tolerance. Our functional characterization of PSK1 adds to global knowledge of the multiple functions of previously explored SKP1-like genes in plants and sheds light on the molecular mechanism underlying its role in salinity tolerance. Our findings also provide information on the function and molecular mechanism of PSK1 in tree peony flower development, thereby revealing a theoretical basis for regulation of flowering and conferral of salinity tolerance in tree peony.
学科主题Plant Sciences
出版地NEW YORK
电子版国际标准刊号1432-203X
WOS关键词SALT TOLERANCE ; SELF-INCOMPATIBILITY ; STRESS TOLERANCE ; MALE MEIOSIS ; PROTEIN ; EXPRESSION ; SKP1 ; FAMILY ; IDENTIFICATION ; ASK1
语种英语
WOS记录号WOS:000403488000001
出版者SPRINGER
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272201, 31471909]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/22031]  
专题中科院北方资源植物重点实验室
作者单位1.Qingdao Agr Univ, Landscape & Forestry Coll, Qingdao 266109, Peoples R China
2.Int Ctr Bamboo & Rattan, Key Lab Sci & Technol Bamboo & Rattan, Beijing 100102, Peoples R China
3.Chinese Acad Sci, Key Lab Plant Resources Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Hao, Qing,Ren, Hongxu,Zhu, Jin,et al. Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis[J]. PLANT CELL REPORTS,2017,36(1):151-162.
APA Hao, Qing.,Ren, Hongxu.,Zhu, Jin.,Wang, Liangsheng.,Huang, Shouchen.,...&Shu, Qingyan.(2017).Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis.PLANT CELL REPORTS,36(1),151-162.
MLA Hao, Qing,et al."Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis".PLANT CELL REPORTS 36.1(2017):151-162.

入库方式： OAI收割

来源：植物研究所

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