Phylogenetic and functional analysis of the basic transcription factor gene BTF3 from Jatropha curcas
文献类型：期刊论文
| 作者 | Peng, Xianjun; Wang, Qi1; Liu, Hui; Shen, Shihua
|
| 刊名 | PLANT GROWTH REGULATION
 |
| 出版日期 | 2017 |
| 卷号 | 82期号:2页码:247-257 |
| 关键词 | Abiotic stress BTF Cold stress Phylogenetic Transcriptional factor |
| ISSN号 | 0167-6903 |
| DOI | 10.1038/srep39886 |
| 文献子类 | Article |
| 英文摘要 | The potential biofuel crop Jatropha curcas has very low tolerance to low temperatures, which is the bottleneck for its cultivation and commercialization. BTF3 (Basic transcription factor 3) function as a key regulator of plant growth and development as well as in the tolerance to biotic and abiotic stress. JcBTF3 was isolated from J. curcas by screening the cold stress responsive yeast hybrid library, which has five introns and six exons that contain a single ORF of 501 bp, encoding a protein of 166 amino acids. In addition to its NAC domain, phylogenetic analysis suggests that the entire sequence of the JcBTF3 protein and the gene structure are highly conserved in the plant kingdom. One hour of cold exposure caused a significant up-regulation of JcBTF3, while abscisic acid, methyl jasmonate, salt and drought stresses slightly induced its expression. Assessing expression in different tissues showed that JcBTF3 is expressed ubiquitously, with lower levels in the stem. Further experiments demonstrated that JcBTF3 is distributed throughout the cytoplasm and in the nucleus. Overexpression of JcBTF3 increased the tolerance to cold stresses in transgenic Arabidopsis. These results suggest that the conserved BTF3 gene structure could be an important hint for the functional identification of orthologous genes in additional plant species and that JcBTF3 might be used to improve the cold stress resistance of J. curcas. |
| 学科主题 | Science & Technology - Other Topics |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5087 |
| WOS关键词 | STRESS-RESPONSIVE PROTEINS ; PROTEOMIC ANALYSIS ; COLD STRESS ; EXPRESSION ; IDENTIFICATION ; SALT ; PHOTOSYNTHESIS ; MITOCHONDRIA ; TOLERANCE ; APOPTOSIS |
| 语种 | 英语 |
| WOS记录号 | WOS:000391547100002 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270653] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22096]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Zheng Zhou Fruit Res Inst, Zhengzhou 450009, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Xianjun,Wang, Qi,Liu, Hui,et al. Phylogenetic and functional analysis of the basic transcription factor gene BTF3 from Jatropha curcas[J]. PLANT GROWTH REGULATION,2017,82(2):247-257. |
| APA | Peng, Xianjun,Wang, Qi,Liu, Hui,&Shen, Shihua.(2017).Phylogenetic and functional analysis of the basic transcription factor gene BTF3 from Jatropha curcas.PLANT GROWTH REGULATION,82(2),247-257. |
| MLA | Peng, Xianjun,et al."Phylogenetic and functional analysis of the basic transcription factor gene BTF3 from Jatropha curcas".PLANT GROWTH REGULATION 82.2(2017):247-257. |
入库方式： OAI收割
来源：植物研究所
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