Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds
文献类型：期刊论文
| 作者 | Yin, Qinggang1; Shen, Guoan; Chang, Zhenzhan5; Tang, Yuhong4; Gao, Hongwen2; Pang, Yongzhen |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2017 |
| 卷号 | 68期号:3页码:594-609 |
| 关键词 | Enzymatic activity flavonols Lotus japonicus seed UDP-glycosyltransferase UGT72 family |
| ISSN号 | 0022-0957 |
| DOI | 10.1016/j.biocon.2017.05.029 |
| 文献子类 | Article |
| 英文摘要 | Flavonols are one of the largest groups of flavonoids that confer benefits for the health of plants and animals. Flavonol glycosides are the predominant flavonoids present in the model legume Lotus japonicus. The molecular mechanisms underlying the biosynthesis of flavonol glycosides as yet remain unknown in L. japonicus. In the present study, we identified a total of 188 UDP-glycosyltransferases (UGTs) in L. japonicus by genome-wide searching. Notably, 12 UGTs from the UGT72 family were distributed widely among L. japonicus chromosomes, expressed in all tissues, and showed different docking scores in an in silico bioinformatics docking analysis. Further enzymatic assays showed that five recombinant UGTs (UGT72AD1, UGT72AF1, UGT72AH1, UGT72V3, and UGT72Z2) exhibit activity toward flavonol, flavone, and isoflavone aglycones. In particular, UGT72AD1, UGT72AH1, and UGT72Z2 are flavonol-specific UGTs with different kinetic properties. In addition, the overexpression of UGT72AD1 and UGT72Z2 led to increased accumulation of flavonol rhamnosides in L. japonicus and Arabidopsis thaliana. Moreover, the increase of kaempferol 3-O-rhamnoside-7-O-rhamnoside in transgenic A. thaliana inhibited root growth as compared with the wild-type control. These results highlight the significance of the UGT72 family in flavonol glycosylation and the role of flavonol rhamnosides in plant growth. |
| 学科主题 | Biodiversity & Conservation ; Environmental Sciences & Ecology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | UDP-GLYCOSYLTRANSFERASES ; FUNCTIONAL-CHARACTERIZATION ; AUXIN TRANSPORT ; IDENTIFICATION ; GENES ; LEGUME ; EXPRESSION ; DIFFERENTIATION ; TRANSCRIPTOME ; GLYCOSYLATION |
| 语种 | 英语 |
| WOS记录号 | WOS:000404709100001 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Major State Basic Research Development ProgramNational Basic Research Program of China [2014CB138703] ; International S&T Cooperation Program of China [2015DFR30570] ; Hundred Talents Program of the Chinese Academy of SciencesChinese Academy of Sciences [39391503-7] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22200]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Samuel Roberts Noble Fdn Inc, Ardmore, OK 73042 USA 3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 4.Peking Univ, Sch Basic Med Sci, Dept Biophys, Beijing 100191, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Qinggang,Shen, Guoan,Chang, Zhenzhan,et al. Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds[J]. JOURNAL OF EXPERIMENTAL BOTANY,2017,68(3):594-609. |
| APA | Yin, Qinggang,Shen, Guoan,Chang, Zhenzhan,Tang, Yuhong,Gao, Hongwen,&Pang, Yongzhen.(2017).Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds.JOURNAL OF EXPERIMENTAL BOTANY,68(3),594-609. |
| MLA | Yin, Qinggang,et al."Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds".JOURNAL OF EXPERIMENTAL BOTANY 68.3(2017):594-609. |
入库方式： OAI收割
来源：植物研究所
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