Heavy metal ATPase 3 (HMA3) confers cadmium hypertolerance on the cadmium/zinc hyperaccumulator Sedum plumbizincicola
文献类型：期刊论文
| 作者 | Liu, Huan2; Zhao, Haixia2; Wu, Longhua1; Liu, Anna; Zhao, Fang-Jie; Xu, Wenzhong |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2017 |
| 卷号 | 215期号:2页码:687-698 |
| ISSN号 | 0028-646X |
| 关键词 | cadmium (Cd) heavy metal ATPase 3 (HMA3) hyperaccumulator Sedum plubizincicola tolerance transgenic plants transporter vacuole |
| DOI | 10.1016/j.biocon.2017.05.011 |
| 文献子类 | Article |
| 英文摘要 | Cadmium (Cd) is highly toxic to most organisms, but some rare plant species can hyperaccumulate Cd in aboveground tissues without suffering from toxicity. The mechanism underlying Cd detoxification by hyperaccumulators is interesting but unclear. Here, the heavy metal ATPase 3 (SpHMA3) gene responsible for Cd detoxification was isolated from the Cd/zinc (Zn) hyperaccumulator Sedum plumbizincicola. RNA interference (RNAi)-mediated silencing and overexpression of SpHMA3 were induced to investigate its physiological functions in S. plumbizincicola and a nonhyperaccumulating ecotype of Sedum alfredii. Heterologous expression of SpHMA3 in Saccharomyces cerevisiae showed Cd-specific transport activity. SpHMA3 was highly expressed in the shoots and the protein was localized to the tonoplast. The SpHMA3-RNAi lines were hypersensitive to Cd but not to Zn, with the growth of shoots and young leaves being severely inhibited by Cd. Overexpressing SpHMA3 in the nonhyperaccumulating ecotype of S. alfredii greatly increased its tolerance to and accumulation of Cd, but not Zn. These results indicate that elevated expression of the tonoplast-localized SpHMA3 in the shoots plays an essential role in Cd detoxification, which contributes to the maintenance of the normal growth of young leaves of S. plumbizincicola in Cd-contaminated soils. |
| 学科主题 | Biodiversity & Conservation ; Environmental Sciences & Ecology |
| 电子版国际标准刊号 | 1469-8137 |
| 出版地 | HOBOKEN |
| WOS关键词 | ARABIDOPSIS-HALLERI ; ALFREDII HANCE ; CD TOLERANCE ; PLANTS ; ZINC ; ACCUMULATION ; EXPRESSION ; GENES ; DETOXIFICATION ; ROLES |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000404809900015 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41325003, 91117010] ; Chinese Academy of SciencesChinese Academy of Sciences [KSCX2-YW-G053] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22217]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Huan,Zhao, Haixia,Wu, Longhua,et al. Heavy metal ATPase 3 (HMA3) confers cadmium hypertolerance on the cadmium/zinc hyperaccumulator Sedum plumbizincicola[J]. NEW PHYTOLOGIST,2017,215(2):687-698. |
| APA | Liu, Huan,Zhao, Haixia,Wu, Longhua,Liu, Anna,Zhao, Fang-Jie,&Xu, Wenzhong.(2017).Heavy metal ATPase 3 (HMA3) confers cadmium hypertolerance on the cadmium/zinc hyperaccumulator Sedum plumbizincicola.NEW PHYTOLOGIST,215(2),687-698. |
| MLA | Liu, Huan,et al."Heavy metal ATPase 3 (HMA3) confers cadmium hypertolerance on the cadmium/zinc hyperaccumulator Sedum plumbizincicola".NEW PHYTOLOGIST 215.2(2017):687-698. |
入库方式： OAI收割
来源：植物研究所
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