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Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.)

文献类型：期刊论文

作者Zheng, Guangshun3,4,5; Fan, Cunying4,5; Di, Shaokang5; Wang, Xuemin3; Xiang, Chengbin2; Pang, Yongzhen3,5
刊名FRONTIERS IN PLANT SCIENCE
出版日期2017
卷号8
关键词alfalfa drought tolerance AtEDT1 gene transgenic alfalfa over-expression
ISSN号1664-462X
DOI10.1016/j.jop.2017.08.005
文献子类Article
英文摘要Alfalfa (Medicago sativa L.) is an important legume forage crop with great economic value. However, as the growth of alfalfa is seriously affected by an inadequate supply of water, drought is probably the major abiotic environmental factor that most severely affects alfalfa production worldwide. In an effort to enhance alfalfa drought tolerance, we transformed the Arabidopsis Enhanced Drought Tolerance 1 (AtEDT1) gene into alfalfa via Agrobacterium-mediated transformation. Compared with wild type plants, drought stress treatment resulted in higher survival rates and biomass, but reduced water loss rates in the transgenic plants. Furthermore, transgenic alfalfa plants had increased stomatal size, but reduced stomatal density, and these stomatal changes contributed greatly to reduced water loss from leaves. Importantly, transgenic alfalfa plants exhibited larger root systems with larger root lengths, root weight, and root diameters than wild type plants. The transgenic alfalfa plants had reduced membrane permeability and malondialdehyde content, but higher soluble sugar and proline content, higher superoxide dismutase activity, higher chlorophyll content, enhanced expression of drought-responsive genes, as compared with wild type plants. Notably, transgenic alfalfa plants grew better in a 2-year field trial and showed enhanced growth performance with increased biomass yield. All of our morphological, physiological, and molecular analyses demonstrated that the ectopic expression of AtEDT1 improved growth and enhanced drought tolerance in alfalfa. Our study provides alfalfa germplasm for use in forage improvement programs, and may help to increase alfalfa production in arid lands.
学科主题Geology ; Paleontology
出版地LAUSANNE
WOS关键词NAC TRANSCRIPTION FACTOR ; CO-TRANSFORMING BAR ; ACTIVATED EXPRESSION ; ENHANCED RESISTANCE ; STRESS TOLERANCE ; SALT TOLERANCE ; OVEREXPRESSION ; YIELD ; COTTON ; ATEDT1/HDG11
语种英语
WOS记录号WOS:000418500000005
出版者FRONTIERS MEDIA SA
资助机构Major State Basic Research and Development Program of ChinaNational Basic Research Program of China [2014CB138703] ; International S&T Cooperation Program of China [2015DFR30570-3]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/22344]  
专题中科院北方资源植物重点实验室
作者单位1.Univ Sci & Technol China, Sch Life Sci, Hefei, Anhui, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China
4.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Guangshun,Fan, Cunying,Di, Shaokang,et al. Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.)[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA Zheng, Guangshun,Fan, Cunying,Di, Shaokang,Wang, Xuemin,Xiang, Chengbin,&Pang, Yongzhen.(2017).Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.).FRONTIERS IN PLANT SCIENCE,8.
MLA Zheng, Guangshun,et al."Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.)".FRONTIERS IN PLANT SCIENCE 8(2017).

入库方式： OAI收割

来源：植物研究所

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