Identified of a novel cis-element regulating the alternative splicing of LcDREB2
文献类型：期刊论文
| 作者 | Liu, Zhujiang1; Yuan, Guangxiao1; Liu, Shu1; Jia, Junting1; Cheng, Liqin; Qi, Dongmei; Shen, Shihua ; Peng, Xianjun; Liu, Gongshe
|
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/hdy.2017.28 |
| 文献子类 | Article |
| 英文摘要 | Alternative splicing (AS) is an important gene regulation mechanism in plants. Despite the widespread use of AS in plant gene expression regulation, the identification of the cis-elements involved in the AS mechanism is rarely reported in plants. To explore the regulation mechanism of the AS of LcDREB2, a DREB2 ortholog from Sheepgrass (Leymus chinensis), the genomic sequences of LcDREB2 and its homologs in Poaceae were aligned, and six mutations were introduced in the conserved sequence of LcDREB2. By analyzing the distinct transcript patterns of the LcDREB2 mutants in transgenic Oryza sativa, a novel cis-element that affected the AS of LcDREB2 was identified as Exonic Splicing Enhancer 1 (ESE1). In addition, five serine-arginine rich (SR) proteins were confirmed to interact with ESE1 by electrophoretic mobility shift assay (EMSA). To further explore the expression regulation mechanism of the DREB subfamily, phylogenetic analysis of DREB2 paralogous genes was performed. The results strongly supported the hypothesis that AS is conserved in Poaceae plants and that it is an evolutionary strategy for the regulation of the functional expression of genes. The findings and methods of our study will promote a substantial step forward in understanding of the plant AS regulation mechanism. |
| 学科主题 | Environmental Sciences & Ecology ; Evolutionary Biology ; Genetics & Heredity |
| 出版地 | LONDON |
| WOS关键词 | RESPONSIVE GENE-EXPRESSION ; ARABIDOPSIS TRANSCRIPTION FACTOR ; PRE-MESSENGER-RNA ; FUNCTIONAL-ANALYSIS ; STRESS TOLERANCE ; BINDING-PROTEIN ; FACTOR DREB2A ; SR PROTEINS ; PLANTS ; LANDSCAPE |
| 语种 | 英语 |
| WOS记录号 | WOS:000407362100007 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470411] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB138704, 973] ; Conservation of Forage Genetic Resources Porgram [2130135] ; Project of Ningxia Agricultural Comprehensive Development Office [NTKJ-2014-04(1)] ; Project of Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-EW-STS-119] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22351]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhujiang,Yuan, Guangxiao,Liu, Shu,et al. Identified of a novel cis-element regulating the alternative splicing of LcDREB2[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Liu, Zhujiang.,Yuan, Guangxiao.,Liu, Shu.,Jia, Junting.,Cheng, Liqin.,...&Liu, Gongshe.(2017).Identified of a novel cis-element regulating the alternative splicing of LcDREB2.SCIENTIFIC REPORTS,7. |
| MLA | Liu, Zhujiang,et al."Identified of a novel cis-element regulating the alternative splicing of LcDREB2".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：植物研究所
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