Characterization of Genes Encoding Key Enzymes Involved in Anthocyanin Metabolism of Kiwifruit during Storage Period
文献类型：期刊论文
| 作者 | Li, Boqiang; Xia, Yongxiu1; Wang, Yuying; Qin, Guozheng ; Tian, Shiping1
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| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2017 |
| 卷号 | 8 |
| 关键词 | red fleshed kiwifruit anthocyanin low temperature molecular basis MYB transcription factor |
| ISSN号 | 1664-462X |
| DOI | 10.1093/gbe/evx180 |
| 文献子类 | Article |
| 英文摘要 | 'Hongyang' is a red fleshed kiwifruit with high anthocyanin content. In this study, we mainly investigated effects of different temperatures (25 and 0 degrees C) on anthocyanin biosynthesis in harvested kiwifruit, and characterized the genes encoding key enzymes involved in anthocyanin metabolism, as well as evaluated the mode of the action, by which low temperature regulates anthocyanin accumulation in 'Hongyang' kiwifruit during storage period. The results showed that low temperature could effectively enhance the anthocyanin accumulation of kiwifruit in the end of storage period (90 days), which related to the increase in mRNA levels of ANS1, ANS2, DRF1, DRF2, and UGFT2. Moreover, the transcript abundance of MYBA1-1 and MYB5-1, the genes encoding an important component of MYB-bHLH-WD40 (MBW) complex, was up-regulated, possibly contributing to the induction of specific anthocyanin biosynthesis genes under the low temperature. To further investigate the roles of AcMYB5-1/5-2/A1-1 in regulation of anthocyanin biosynthesis, genes encoding the three transcription factors were transiently transformed in Nicotiana benthamiana leaves. Overexpression of AcMYB5-1/5-2/A1-1 activated the gene expression of NtANS and NtDFR in tobacco. Our results suggested that low temperature storage could stimulate the anthocyanin accumulation in harvested kiwifruit via regulating several structural and regulatory genes involved in anthocyanin biosynthesis. |
| 学科主题 | Evolutionary Biology ; Genetics & Heredity |
| 出版地 | LAUSANNE |
| WOS关键词 | RED-FLESHED KIWIFRUIT ; ACTINIDIA-CHINENSIS ; HIGH-TEMPERATURE ; GRAPE BERRIES ; APPLE SKIN ; BIOSYNTHESIS ; FRUIT ; TRANSCRIPTION ; ACCUMULATION ; EXPRESSION |
| 语种 | 英语 |
| WOS记录号 | WOS:000412147400015 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2016YFD0400902] ; Youth Innovation Promotion Association CAS [2015063] ; Open Fund of Key Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), State Ethnic Affairs Commission & Ministry of Education, China |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22352]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Boqiang,Xia, Yongxiu,Wang, Yuying,et al. Characterization of Genes Encoding Key Enzymes Involved in Anthocyanin Metabolism of Kiwifruit during Storage Period[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Li, Boqiang,Xia, Yongxiu,Wang, Yuying,Qin, Guozheng,&Tian, Shiping.(2017).Characterization of Genes Encoding Key Enzymes Involved in Anthocyanin Metabolism of Kiwifruit during Storage Period.FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Li, Boqiang,et al."Characterization of Genes Encoding Key Enzymes Involved in Anthocyanin Metabolism of Kiwifruit during Storage Period".FRONTIERS IN PLANT SCIENCE 8(2017). |
入库方式： OAI收割
来源：植物研究所
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