BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance
文献类型：期刊论文
| 作者 | Wang, Bo1; Du, Hong2; Zhang, Zhennan; Xu, Wenzhong; Deng, Xin
|
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2017 |
| 卷号 | 8 |
| 关键词 | Arabidopsis Boea hygrometrica bZIP60 drought tolerance ER-QC mRNA-splicing UPR |
| ISSN号 | 1664-462X |
| DOI | 10.1016/j.palwor.2017.01.006 |
| 文献子类 | Article |
| 英文摘要 | Adverse environmental conditions cause endoplasmic reticulum (ER) stress in plants. To mitigate ER stress damage, ER associated transcription factors and inositol-requiring enzyme-1 (IRE1)-mediated bZIP60 mRNA splicing are activated in plants. A drought-induced gene, encoding the ortholog of AtbZIP60, was identified in the resurrection plant Boea hygrometrica, termed BhbZIP60. In response to ER stress and dehydration, BhbZIP60 mRNA can be spliced to create a frame shift in the C terminus by the excision of 23b segment in a manner of its ortholog in other plants, thus translocating to the nucleus instead of the cytoplasm. The splicing-activated BhbZIP60 (BhbZIP60S) could function in the same way as its Arabidopsis ortholog by restoring the molecular phenotype of the mutant atbzip60. When overexpressed in Arabidopsis, BhbZIP60S provided transgenic plants with enhanced tolerance to drought, tunicamycin and mannitol stresses with upregulation of the expressions of ER quality control (QC) genes (BiP2, BiP3, CNX1, and sPDI) and abscisic acid (ABA) responsive genes (RD29A, RAB18, and RD17). Furthermore, in the yeast one-hybrid system, BhbZIP60S was capable of interacting with ER stress responsive elements (ERSE and ERSE-II) that exist in the promoters of known ER-QC genes, but not binding to ABA responsive ciselements (ABREs). Our results demonstrated that drought-induced BhbZIP60 may have a function in drought tolerance via the splicing-activated BhbZIP60S to mediate ER-QC by direct binding to the promoters of ER-QC genes. This study evidently demonstrates the involvement of ER-QC in the drought tolerance of Arabidopsis and the desiccation tolerance of the resurrection plant B. hygrometrica. |
| 学科主题 | Paleontology |
| 出版地 | LAUSANNE |
| WOS关键词 | UNFOLDED PROTEIN RESPONSE ; BZIP TRANSCRIPTION FACTOR ; QUALITY-CONTROL ; ER-STRESS ; SIGNAL-TRANSDUCTION ; ABSCISIC-ACID ; ARABIDOPSIS ; GENES ; ABA ; DEHYDRATION |
| 语种 | 英语 |
| WOS记录号 | WOS:000408673600011 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300230, 31270312, 31470361] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2012CB114302] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22354]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Xinjiang Agr Univ, Coll Agr, Urumqi, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Bo,Du, Hong,Zhang, Zhennan,et al. BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Wang, Bo,Du, Hong,Zhang, Zhennan,Xu, Wenzhong,&Deng, Xin.(2017).BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance.FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Wang, Bo,et al."BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance".FRONTIERS IN PLANT SCIENCE 8(2017). |
入库方式： OAI收割
来源：植物研究所
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