Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)
文献类型：期刊论文
| 作者 | Liu, Ruiling2; Li, Boqiang; Qin, Guozheng ; Zhang, Zhanquan; Tian, Shiping2
|
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2017 |
| 卷号 | 8 |
| 关键词 | tomato tonoplast transporters overexpression RNAi organic acid composition |
| ISSN号 | 1664-462X |
| DOI | 10.11646/phytotaxa.316.2.2 |
| 文献子类 | Article |
| 英文摘要 | Acidity plays an important role in flavor and overall organoleptic quality of fruit and is mainly due to the presence of organic acids. Understanding the molecular basis of organic acid metabolism is thus of primary importance for fruit quality improvement. Here, we cloned a putative tonoplast dicarboxylate transporter gene (SlTDT) from tomato, and submitted it to the NCBI database (GenBank accession number: KC733165). SlTDT protein contained 13 putative transmembrane domains in silico analysis. Confocal microscopic study using green fluorescent fusion proteins revealed that SlTDT was localized on tonoplast. The expression patterns of SlTDT in tomato were analyzed by RT-qPCR. The results indicated that SlTDT expressed in leaves, roots, flowers and fruits at different ripening stages, suggesting SlTDT may be associated with the development of different tissues. To further explore the function of SlTDT, we constructed both overexpression and RNAi vectors and obtained transgenic tomato plants by agrobacterium-mediated method. Gas chromatographymass spectrometer (GC-MS) analysis showed that overexpression of SlTDT significantly increased malate content, and reduced citrate content in tomato fruit. By contrast, repression of SlTDT in tomato reduced malate content of and increased citrate content. These results indicated that SlTDT played an important role in remobilization of malate and citrate in fruit vacuoles. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | FRUIT-DEVELOPMENT ; APPLE FRUIT ; ORGANIC-ACIDS ; MALATE ; METABOLISM ; EXPRESSION ; ACCUMULATION ; CHANNEL ; MEMBRANE ; BERRIES |
| 语种 | 英语 |
| WOS记录号 | WOS:000406967500002 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Key Research and Development Program of China [2016YFD0400902] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057] ; Youth Innovation Promotion Association CAS [2015063] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/22355]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ruiling,Li, Boqiang,Qin, Guozheng,et al. Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)[J]. FRONTIERS IN PLANT SCIENCE,2017,8. |
| APA | Liu, Ruiling,Li, Boqiang,Qin, Guozheng,Zhang, Zhanquan,&Tian, Shiping.(2017).Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum).FRONTIERS IN PLANT SCIENCE,8. |
| MLA | Liu, Ruiling,et al."Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)".FRONTIERS IN PLANT SCIENCE 8(2017). |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。