A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening
文献类型：期刊论文
| 作者 | Li, Xuyan; Hou, Yanming; Xie, Xin; Li, Hongxue; Li, Xiaodong2; Zhu, Yan2; Zhai, Lulu; Zhang, Chunyu; Bian, Shaomin |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2020 |
| 卷号 | 71期号:19页码:5976-5989 |
| 关键词 | Anthocyanin biosynthesis blueberry chlorophyll accumulation color change fruit development and maturation miR156-SPL module |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/eraa327 |
| 文献子类 | Article |
| 英文摘要 | Color change is an important event during fruit maturation in blueberry, usually depending on chlorophyll degradation and anthocyanin accumulation. MicroRNA156 (miR156)-SPL modules are an important group of regulatory hubs involved in the regulation of anthocyanin biosynthesis. However, little is known regarding their roles in blueberry or in chlorophyll metabolism during color change. In this study, a MIR156 gene (VcMIR156a) was experimentally identified in blueberry (Vaccinium corymbosum). Overexpression of VcMIR156a in tomato (Solanum lycopersicum) enhanced anthocyanin biosynthesis and chlorophyll degradation in the stem by altering pigment-associated gene expression. Further investigation indicated that the VcSPL12 transcript could be targeted by miR156, and showed the reverse accumulation patterns during blueberry fruit development and maturation. Noticeably, VcSPL12 was highly expressed at green fruit stages, while VcMIR156a transcripts mainly accumulated at the white fruit stage when expression of VcSPL12 was dramatically decreased, implying that VcMIR156a-VcSPL12 is a key regulatory hub during fruit coloration. Moreover, VcSPL12 decreased the expression of several anthocyanin biosynthetic and regulatory genes, and a yeast two-hybrid assay indicated that VcSPL12 interacted with VcMYBPA1. Intriguingly, expression of VcSPL12 significantly enhanced chlorophyll accumulation and altered the expression of several chlorophyll-associated genes. Additionally, the chloroplast ultrastructure was altered by the expression of VcMIR156a and VcSPL12. These findings provide a novel insight into the functional roles of miR156-SPLs in plants, especially in blueberry fruit coloration. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | ABSCISIC-ACID ; CHLOROPLAST DEVELOPMENT ; NEGATIVE REGULATION ; GENE-EXPRESSION ; TOMATO FRUIT ; ARABIDOPSIS ; BIOSYNTHESIS ; ACTIVATION ; DEGRADATION ; SENESCENCE |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000577075400023 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872075] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/21560]  |
| 专题 | 植物园 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Jilin Univ, Coll Plant Sci, Changchun 130062, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xuyan,Hou, Yanming,Xie, Xin,et al. A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening[J]. JOURNAL OF EXPERIMENTAL BOTANY,2020,71(19):5976-5989. |
| APA | Li, Xuyan.,Hou, Yanming.,Xie, Xin.,Li, Hongxue.,Li, Xiaodong.,...&Bian, Shaomin.(2020).A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening.JOURNAL OF EXPERIMENTAL BOTANY,71(19),5976-5989. |
| MLA | Li, Xuyan,et al."A blueberry MIR156a-SPL12 module coordinates the accumulation of chlorophylls and anthocyanins during fruit ripening".JOURNAL OF EXPERIMENTAL BOTANY 71.19(2020):5976-5989. |
入库方式： OAI收割
来源：植物研究所
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