Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy
文献类型：期刊论文
| 作者 | Zhu, Sen1; Li, Xuan2; Dang, Bingrong3 ; Wu, Fen1; Wang, Chunming1; Lin, Changjun1
|
| 刊名 | REDOX REPORT
 |
| 出版日期 | 2022-12-31 |
| 卷号 | 27期号:1页码:32-44 |
| ISSN号 | 1351-0002 |
| 关键词 | PM2 5 Lycium barbarum polysaccharide (LBP) antioxidant oxidative damage endoplasmic reticulum (ER) stress autophagy apoptosis mitochondrial damage |
| DOI | 10.1080/13510002.2022.2036507 |
| 通讯作者 | Lin, Changjun(linc@lzu.edu.cn) |
| 英文摘要 | Objectives: Lycium barbarum polysaccharide (LBP) is a natural polysaccharide extracted from Lycium barbarum that has anti-inflammatory, anti-apoptotic and anti-aging effects, and plays a role in the prevention and treatment of various diseases. In this study, we investigated the therapeutic effect of LBP on particulate matter 2.5 (PM2.5)-induced skin damage. Methods: Cell viability was analyzed by MTT and LDH assays. Apoptosis was analyzed by Annexin V-FITC/PI staining. Oxidative stress/damage were assessed by intracellular ROS levels, MDA content and SOD activity. The intracellular protein expression was analyzed by Western blot. Mitochondrial damage was assayed by mitochondrial membrane potential with JC-1 probe. LC3-GFP adenovirus was transfected into HaCaT cells to analyze intracellular autophagosome levels. Results: In PM2.5-treated HaCaT cells, LBP pretreatment reduced PM2.5-induced cytotoxicity, ameliorated cell morphology and reduced cell apoptosis. LBP also inhibited the expression levels of GRP78 and CHOP, reduced the conversion of LC3I to LC3II, inhibited Bax protein and activated Bcl-2 protein. Furthermore, LBP inhibited PM2.5-induced mitochondrial autophagy (mitophagy) and mitochondrial damage. PM2.5-induced autophagy was regulated by endoplasmic reticulum (ER) stress. Conclusion: LBP protects skin cells from PM2.5-induced cytotoxicity by regulating the oxidative stress-ER stress-autophagy-apoptosis signaling axis, revealing that LBP has a great potential for the skin protection. |
| WOS关键词 | KERATINOCYTES ; CANCER ; DAMAGE |
| 资助项目 | LZU-IMPCAS cooperation |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS LTD |
| WOS记录号 | WOS:000752638300001 |
| 资助机构 | LZU-IMPCAS cooperation |
| 源URL | [http://119.78.100.186/handle/113462/142135]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Lin, Changjun |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Lanzhou Univ Second Hosp, Lanzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Sen,Li, Xuan,Dang, Bingrong,et al. Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy[J]. REDOX REPORT,2022,27(1):32-44. |
| APA | Zhu, Sen,Li, Xuan,Dang, Bingrong,Wu, Fen,Wang, Chunming,&Lin, Changjun.(2022).Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy.REDOX REPORT,27(1),32-44. |
| MLA | Zhu, Sen,et al."Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy".REDOX REPORT 27.1(2022):32-44. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。