Circulating tRNA-Derived Small RNAs as Novel Radiation Biomarkers of Heavy Ion, Proton and X-ray Exposure
文献类型：期刊论文
| 作者 | Wei, Wenjun1,2,3 ; Bai, Hao1,2,3; Chen, Yaxiong1,2; Zhang, Tongshan1,2,3; Zhang, Yanan1,2; Hua, Junrui1,2; He, Jinpeng1,2,3; Ding, Nan1,2,3; Zhou, Heng1,2,3; Wang, Jufang1,2,3
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2021-12-01 |
| 卷号 | 22期号:24页码:15 |
| 关键词 | tsRNA serum biomarkers radiation exposure multi-factor models |
| DOI | 10.3390/ijms222413476 |
| 通讯作者 | Wang, Jufang() |
| 英文摘要 | The effective and minimally invasive radiation biomarkers are valuable for exposure scenarios in nuclear accidents or space missions. Recent studies have opened the new sight of circulating small non-coding RNA (sncRNA) as radiation biomarkers. The tRNA-derived small RNA (tsRNA) is a new class of sncRNA. It is more abundant than other kinds of sncRNAs in extracellular vesicles or blood, presenting great potential as promising biomarkers. However, the circulating tsRNAs in response to ionizing radiation have not been reported. In this research, Kunming mice were total-body exposed to 0.05-2 Gy of carbon ions, protons, or X-rays, and the RNA sequencing was performed to profile the expression of sncRNAs in serum. After conditional screening and validation, we firstly identified 5 tsRNAs including 4 tRNA-related fragments (tRFs) and 1 tRNA half (tiRNA) which showed a significant level decrease after exposure to three kinds of radiations. Moreover, the radiation responses of these 5 serum tsRNAs were reproduced in other mouse strains, and the sequences of them could be detected in serum of humans. Furthermore, we developed multi-factor models based on tsRNA biomarkers to indicate the degree of radiation exposure with high sensitivity and specificity. These findings suggest that the circulating tsRNAs can serve as new minimally invasive biomarkers and can make a triage or dose assessment from blood sample collection within 4 h in exposure scenarios. |
| WOS关键词 | BLOOD-LYMPHOCYTES ; FRAGMENTS ; MICRORNA ; SERUM ; BIODOSIMETRY ; CANCER |
| 资助项目 | National Natural Science Foundation of China[11805246] ; National Natural Science Foundation of China[12175289] ; National Natural Science Foundation of China[31870851] ; Science and Technology Research Project of Gansu Province[145RTSA012] ; CAS Light of West China program ; fund of innovation center of radiation application[KFZC2020010701] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000738673800001 |
| 出版者 | MDPI |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Research Project of Gansu Province ; CAS Light of West China program ; fund of innovation center of radiation application |
| 源URL | [http://119.78.100.186/handle/113462/142437]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Wang, Jufang |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, CAS Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Inst Modern Phys, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wenjun,Bai, Hao,Chen, Yaxiong,et al. Circulating tRNA-Derived Small RNAs as Novel Radiation Biomarkers of Heavy Ion, Proton and X-ray Exposure[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(24):15. |
| APA | Wei, Wenjun.,Bai, Hao.,Chen, Yaxiong.,Zhang, Tongshan.,Zhang, Yanan.,...&Wang, Jufang.(2021).Circulating tRNA-Derived Small RNAs as Novel Radiation Biomarkers of Heavy Ion, Proton and X-ray Exposure.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(24),15. |
| MLA | Wei, Wenjun,et al."Circulating tRNA-Derived Small RNAs as Novel Radiation Biomarkers of Heavy Ion, Proton and X-ray Exposure".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.24(2021):15. |
入库方式： OAI收割
来源：近代物理研究所
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