Microfluidic-based in vitro thrombosis model for studying microplastics toxicity
文献类型：期刊论文
| 作者 | Chen, Longfei6,7; Zheng, Yajing1; Liu, Yantong6,7; Tian, Pengfu6; Yu, Le6; Bai, Long4; Zhou, Fuling3; Yang, Yi6,7; Cheng, Yanxiang1; Wang, Fubing2 |
| 刊名 | LAB ON A CHIP
 |
| 出版日期 | 2022-02-09 |
| 页码 | 10 |
| ISSN号 | 1473-0197 |
| DOI | 10.1039/d1lc00989c |
| 通讯作者 | Yang, Yi(yangyiys@whu.edu.cn) ; Cheng, Yanxiang(yanxiangCheng@whu.edu.cn) |
| 英文摘要 | The potential impact of microplastics (MPs) on health has caused great concern, and a toxicology platform that realistically reproduces the system behaviour is urgently needed to further explore and validate MP-related health issues. Herein, we introduce an optically assisted thrombus platform to reveal the interaction of MPs with the vascular system. The risk of accumulation has also been evaluated using a mouse model, and the effect of MPs on the properties of the thrombus are validated via in vitro experiments. The microfluidic system is endothelialized, and the regional tissue injury-induced thrombosis is then realized through optical irradiation. Whole blood is perfused with MPs, and the invasion process visualized and recorded. The mouse model shows a cumulative risk in the blood with continuous exposure to MPs (P-value < 0.0001). The on-chip results show that MP invasion leads to decreased binding of fibrin to platelets (P-value < 0.0001), which is consistent with the results of the in vitro experiments, and shows a high risk of thrombus shedding in real blood flow compared with normal thrombus. This work provides a new method to further reveal MP-related health risks. |
| 资助项目 | National Key R&D Program of China[2018YFC1003200] ; National Natural Science Foundation of China[62175190] ; National Natural Science Foundation of China[11774274] ; National Natural Science Foundation of China[81860276] ; National Natural Science Foundation of China[81770179] ; Foundation Research Fund of Shenzhen Science and Technology Program[JCYJ20190808154409678] ; Support Projects of Medical Science and Technology Innovation Platform of Wuhan University Zhongnan Hospital in 2020[lcyf202010] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000757486500001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178056]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Yang, Yi; Cheng, Yanxiang |
| 作者单位 | 1.Wuhan Univ, Dept Obstet & Gynecol, Renmin Hosp, Wuhan 430060, Peoples R China 2.Wuhan Univ, Zhongnan Hosp, Dept Lab Med, Wuhan 430071, Peoples R China 3.Wuhan Univ, Zhongnan Hosp, Dept Hematol, Wuhan 430071, Peoples R China 4.Zhejiang Univ, Sch Med, Hangzhou 310002, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266061, Peoples R China 6.Wuhan Univ, Sch Phys & Technol, Key Lab Artificial Micro & Nanostruct, Minist Educ, Wuhan 430072, Peoples R China 7.Wuhan Univ, Shenzhen Res Inst, Shenzhen 518000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Longfei,Zheng, Yajing,Liu, Yantong,et al. Microfluidic-based in vitro thrombosis model for studying microplastics toxicity[J]. LAB ON A CHIP,2022:10. |
| APA | Chen, Longfei.,Zheng, Yajing.,Liu, Yantong.,Tian, Pengfu.,Yu, Le.,...&Zhu, Yimin.(2022).Microfluidic-based in vitro thrombosis model for studying microplastics toxicity.LAB ON A CHIP,10. |
| MLA | Chen, Longfei,et al."Microfluidic-based in vitro thrombosis model for studying microplastics toxicity".LAB ON A CHIP (2022):10. |
入库方式： OAI收割
来源：海洋研究所
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