Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency
文献类型：期刊论文
| 作者 | Yu, Wenjie1,4 ; Zhang, Litao2,4; Zhao, Jing4; Liu, Jianguo2,3,4
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| 刊名 | BIORESOURCE TECHNOLOGY
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| 出版日期 | 2022-02-01 |
| 卷号 | 345页码:10 |
| 关键词 | Oxaloacetate Astaxanthin accumulation Haematococcus pluvialis Nitrogen deficiency Respiratory metabolism |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2021.126484 |
| 通讯作者 | Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | Effective inducers and stress conditions play an essential role in the regulation of astaxanthin biosynthesis. This study reports a strategy developed by combining exogenous addition of oxaloacetate (OA) with nitrogen deficiency to facilitate astaxanthin production in Haematococcus pluvialis. Significantly, addition of 10 mM-OA enhanced the cellular astaxanthin content about 7.18-fold under nitrogen deficiency on day 7, with the content of astaxanthin esters increased concomitantly. To further elucidate the role and mechanism of OA on astaxanthin synthesis, the physiological and metabolic analyses of H. pluvialis treated with exogenous OA were performed. The results showed that exogenous OA promoted respiration over photosynthesis. Concurrently, the metabolite levels in the Embden-Meyerhof-Parnas pathway, pentose phosphate pathway and tricarboxylic acid cycle obviously increased. The enhancement of respiratory metabolic pathways led to elevated levels of substrates, thus directly promoted astaxanthin synthesis. The present findings provide a new and effective approach for optimizing astaxanthin production. |
| 资助项目 | National Natural Science Foundation of China[31872588] ; National Natural Science Foundation of China[U1706209] ; TaiShan Industrial Experts Programme[tscy20200102] |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000732933200013 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177554]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Qingdao Langyatai Grp Co Ltd, Shandong Engn Technol Collaborat Innovat Ctr Edib, Qingdao 266400, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Wenjie,Zhang, Litao,Zhao, Jing,et al. Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency[J]. BIORESOURCE TECHNOLOGY,2022,345:10. |
| APA | Yu, Wenjie,Zhang, Litao,Zhao, Jing,&Liu, Jianguo.(2022).Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency.BIORESOURCE TECHNOLOGY,345,10. |
| MLA | Yu, Wenjie,et al."Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency".BIORESOURCE TECHNOLOGY 345(2022):10. |
入库方式： OAI收割
来源：海洋研究所
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