A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis
文献类型：期刊论文
| 作者 | Zhang, Litao1,3,4; Zhang, Chunhui3,4 ; Xu, Ran3,4; Yu, Wenjie3,4; Liu, Jianguo1,2,3,4
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| 刊名 | BIORESOURCE TECHNOLOGY
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| 出版日期 | 2022 |
| 卷号 | 344页码:9 |
| 关键词 | Alternative oxidase Astaxanthin Fatty acid Haematococcus pluvialis Stimulator |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2021.126275 |
| 通讯作者 | Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | In the present study, the mechanisms for facilitating fatty acid and astaxanthin biosynthesis-related processes by inhibiting the alternative oxidase (AOX) respiratory pathway in Haematococcus pluvialis was investigated. The restriction of the AOX pathway induced the accumulation of reactive oxygen species, NAD(P)H and its substrates (acetyl-CoA, pyruvate and glyceraldehy-3-phosphate), which are required for fatty acid and astaxanthin production, thereby promoting the carbon flux into fatty acid and astaxanthin biosynthesis. During a 9-day incubation period, the fatty acid and astaxanthin contents increased by 20.6% and 20.7%, respectively, when the AOX pathway was inhibited approximately 37.7%. The AOX pathway may be inhibited by nutrient (nitrogen and phosphorus) removal, inhibitor addition and air/CO2 aeration adjustments in the large-scale cultivation of H. pluvialis. Therefore, the current study provides a useful enhancement strategy for fatty acid and astaxanthin coproduction and elucidates the roles of the AOX pathway in regulating fatty acid and astaxanthin biosynthesis. |
| 资助项目 | National Key Research and Development Program of China[2018YFE0107200] ; National Natural Science Foundation of China[31872588] ; National Natural Science Foundation of China[U1706209] ; Major Achievement Support Plan Project of LMBB[M019NO01] ; Shandong Provincial Natural Science Foundation, China[ZR2019BC057] |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000732830900011 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177572]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Qingdao Langyatai Grp Co Ltd, Shandong Engn Technol Collaborat Innovat Ctr Edib, Qingdao 266400, Peoples R China 3.Chinese Acad Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Litao,Zhang, Chunhui,Xu, Ran,et al. A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis[J]. BIORESOURCE TECHNOLOGY,2022,344:9. |
| APA | Zhang, Litao,Zhang, Chunhui,Xu, Ran,Yu, Wenjie,&Liu, Jianguo.(2022).A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis.BIORESOURCE TECHNOLOGY,344,9. |
| MLA | Zhang, Litao,et al."A strategy for promoting carbon flux into fatty acid and astaxanthin biosynthesis by inhibiting the alternative oxidase respiratory pathway in Haematococcus pluvialis".BIORESOURCE TECHNOLOGY 344(2022):9. |
入库方式： OAI收割
来源：海洋研究所
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