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Study on artificial induction and early development of gynogenetic fat greenling Hexagrammos otakii

文献类型：期刊论文

作者Wu, Zhihao4,5,6; Tan, Xungang4,5,6; Wang, Lijuan4,5,6; Song, Zongcheng3; Wu, Qiaowan2,4,5,6; Wang, Ling2,4,5,6; Yu, Zhouxin1; Liu, Haoming3; You, Feng4,5,6
刊名AQUACULTURE REPORTS
出版日期2022-02-01
卷号22页码:9
ISSN号2352-5134
关键词Fat greenling Hexagrammos otakii Artificial gynogenesis induction Early growth Genetic diversity Egg envelop structure
DOI10.1016/j.aqrep.2021.100975
通讯作者You, Feng(youfeng@qdio.ac.cn)
英文摘要Fat greenling Hexagrammos otakii, belonging to Scorpaeniformes, is an important commercial marine fish species distributed in coast waters of China, Japan, and Korea. Artificial gynogenesis induction is one of the important techniques of chromosome manipulation and genetic breeding. The eggs are fertilized with genetic inactivated sperm, and genome information of the offspring is totally contributed by maternal genome. Until now, no gynogenesis induction was reported in H. otakii and other Scorpaeniformes species. The present study developed an effective gynogenesis induction method in H. otakii by applying hydrostatic pressure to the eggs activated with UV-irradiated homologous sperm. The optimal inducing conditions were 36,000 erg mm-2 for UV-irradiated and pressure of 65 MPa for 6 min at 20 min post fertilization. The relative hatching rates could reach 92%. The early development and growth before 6 month post hatching showed no difference between the gynogenetic and control diploid. The all-maternal inheritance was confirmed with 4 microsatellite loci. The genetic diversity of the control and gynogenetic diploid revealed with 20 microsatellite loci showed that the homozygosity of the gynogenetic diploid stocks was 0.84 in average, which was nearly double the value of the control diploid stocks (0.45 in average). Under scanning electron microscope, the egg envelop had a gelatinous-honeycomb before 0.5 h post fertilization (hpf). Then all or part of the gelatinous-honeycomb layer disappeared before 24 hpf. The uniform pores distributed to hexagonal pattern on the egg envelop were found in the control diploid and haploid groups. However, no pore on the egg envelop was found in the gynogenetic diploid from fertilization to hatching. These results would provide a valuable reference for gynogenesis induction in fish with viscous ovarian fluid coated eggs, although special incubation process to increase the survival rate should be further developed based on the characteristics of gynogenetic H. otakii.
资助项目National Key Research and Development Program of China[2018YFD0901202] ; National Marine Genetic Resource Center
WOS研究方向Fisheries
语种英语
出版者ELSEVIER
WOS记录号WOS:000735304200001
源URL[http://ir.qdio.ac.cn/handle/337002/177590]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者You, Feng
作者单位1.Qingdao Agr Univ, Qingdao 266237, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
3.Shenghang Ocean Sci & Technol Co Ltd, Weihai 264200, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
6.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Zhihao,Tan, Xungang,Wang, Lijuan,et al. Study on artificial induction and early development of gynogenetic fat greenling Hexagrammos otakii[J]. AQUACULTURE REPORTS,2022,22:9.
APA Wu, Zhihao.,Tan, Xungang.,Wang, Lijuan.,Song, Zongcheng.,Wu, Qiaowan.,...&You, Feng.(2022).Study on artificial induction and early development of gynogenetic fat greenling Hexagrammos otakii.AQUACULTURE REPORTS,22,9.
MLA Wu, Zhihao,et al."Study on artificial induction and early development of gynogenetic fat greenling Hexagrammos otakii".AQUACULTURE REPORTS 22(2022):9.

入库方式： OAI收割

来源：海洋研究所

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