A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria
文献类型：期刊论文
| 作者 | Luo, Jing-Chang1,2,3 ; Zhang, Jian4; Sun, Li1,2,3
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 26期号:24页码:14 |
| 关键词 | g-type lysozyme shrimp deep-sea hydrothermal vent non-enzymatic antibacterial activity |
| DOI | 10.3390/molecules26247624 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Lysozyme is a key effector molecule of the innate immune system in both vertebrate and invertebrate. It is classified into six types, one of which is the goose-type (g-type). To date, no study on g-type lysozyme in crustacean has been documented. Here, we report the identification and characterization of a g-type lysozyme (named LysG1) from the shrimp inhabiting a deep-sea hydrothermal vent in Manus Basin. LysG1 possesses conserved structural features of g-type lysozymes. The recombinant LysG1 (rLysG1) exhibited no muramidase activity and killed selectively Gram-negative bacteria in a manner that depended on temperature, pH, and metal ions. rLysG1 bound target bacteria via interaction with bacterial cell wall components, notably lipopolysaccharide (LPS), and induced cellular membrane permeabilization, which eventually caused cell lysis. The endotoxin-binding capacity enabled rLysG1 to alleviate the inflammatory response induced by LPS. Mutation analysis showed that the bacterial binding and killing activities of rLysG1 required the integrity of the conserved alpha 3 and 4 helixes of the protein. Together, these results provide the first insight into the activity and working mechanism of g-type lysozyme in crustacean and deep-sea organisms. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; CAS Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000736322800001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177623]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 4.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Jing-Chang,Zhang, Jian,Sun, Li. A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria[J]. MOLECULES,2021,26(24):14. |
| APA | Luo, Jing-Chang,Zhang, Jian,&Sun, Li.(2021).A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria.MOLECULES,26(24),14. |
| MLA | Luo, Jing-Chang,et al."A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria".MOLECULES 26.24(2021):14. |
入库方式： OAI收割
来源：海洋研究所
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