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A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection

文献类型：期刊论文

作者Sun, Mingzhe3,4; Li, Shihao1,2,3,4; Jin, Songjun3,4; Li, Xuechun5; Xiang, Jianhai2,3,4; Li, Fuhua1,2,3,4,6
刊名FRONTIERS IN IMMUNOLOGY
出版日期2022-02-23
卷号13页码:12
关键词TRIM9 lymphoid organ IMD signaling pathway WSSV Litopenaeus vannamei
ISSN号1664-3224
DOI10.3389/fimmu.2022.819881
通讯作者Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要The TRIpartite Motif (TRIM) proteins play key roles in cell differentiation, apoptosis, development, autophagy, and innate immunity in vertebrates. In the present study, a novel TRIM9 homolog (designated as LvTRIM9-1) specifically expressed in the lymphoid organ of shrimp was identified from the Pacific whiteleg shrimp Litopenaeus vannamei. Its deduced amino acid sequence possesses the typical features of TRIM proteins, including a RING domain, two B-boxes, a coiled-coil domain, a FN3 domain, and a SPRY domain. The transcripts of LvTRIM9-1 were mainly located in the lymphoid tubules of the lymphoid organ. Knockdown of LvTRIM9-1 could apparently inhibit the transcriptions of some genes from white spot syndrome virus (WSSV) and reduce the viral propagation in the lymphoid organ. Overexpression of LvTRIM9-1 in mammalian cells could activate the promoter activity of NF-kappa B, and an in vivo experiment in shrimp showed that knockdown of LvTRIM9-1 reduced the expression of LvRelish in the lymphoid organ. Yeast two-hybridization and co-immunoprecipitation (Co-IP) assays confirmed that LvTRIM9-1 could directly interact with LvIMD, a key component of the IMD pathway, through its SPRY domain. These data suggest that LvTRIM9-1 could activate the IMD pathway in shrimp via interaction with LvIMD. This is the first evidence to show the regulation of a TRIM9 protein on the IMD pathway through its direct interaction with IMD, which will enrich our knowledge on the role of TRIM proteins in innate immunity of invertebrates.
WOS研究方向Immunology
语种英语
WOS记录号WOS:000766533800001
出版者FRONTIERS MEDIA SA
源URL[http://ir.qdio.ac.cn/handle/337002/178143]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Shihao; Li, Fuhua
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
4.Chinese Acad Sci, Qingdao, Peoples R China
5.Univ Chinese Acad Sci, Beijing, Peoples R China
6.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Mingzhe,Li, Shihao,Jin, Songjun,et al. A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection[J]. FRONTIERS IN IMMUNOLOGY,2022,13:12.
APA Sun, Mingzhe,Li, Shihao,Jin, Songjun,Li, Xuechun,Xiang, Jianhai,&Li, Fuhua.(2022).A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection.FRONTIERS IN IMMUNOLOGY,13,12.
MLA Sun, Mingzhe,et al."A Novel TRIM9 Protein Promotes NF-kappa B Activation Through Interacting With LvIMD in Shrimp During WSSV Infection".FRONTIERS IN IMMUNOLOGY 13(2022):12.

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来源：海洋研究所

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