Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity
文献类型：期刊论文
| 作者 | Wu, Meng1,2,3,4; Jia, Bei-bei1,2,3,4; Li, Mo-fei1,2,3
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| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2022-02-25 |
| 卷号 | 13页码:14 |
| 关键词 | C3 C3a complement Japanese flounder bacterial infection |
| ISSN号 | 1664-3224 |
| DOI | 10.3389/fimmu.2022.813173 |
| 通讯作者 | Li, Mo-fei(murphy210@163.com) |
| 英文摘要 | In the complement system, C3 is a central component in complement activation, immune defense and immune regulation. In all pathways of complement activation, the pivotal step is conversion of the component C3 to C3b and C3a, which is responsible to eliminate the pathogen and opsonization. In this study, we examined the immunological properties of C3 and its activated fragment C3a from Japanese flounder (Paralichthys olivaceus) (PoC3 and PoC3a), a teleost species with important economic value. PoC3 is composed of 1655 amino acid residues, contains the six domains and highly conserved GCGEQ sequence of the C3 family. We found that PoC3 expression occurred in nine different tissues and was upregulated by bacterial challenge. In serum, PoC3 was able to bind to a broad-spectrum of bacteria, and purified native PoC3 could directly kill specific pathogen. When PoC3 expression in Japanese flounder was knocked down by siRNA, serum complement activity was significantly decreased, and bacterial replication in fish tissues was significantly increased. Recombinant PoC3a (rPoC3a) exhibited apparent binding capacities to bacteria and Japanese flounder peripheral blood leukocytes (PBL) and induce chemotaxis of PBL. Japanese flounder administered rPoC3a exhibited enhanced resistance against bacterial infection. Taken together, these results indicate that PoC3 is likely a key factor of complement activation, and PoC3 and PoC3a are required for optimal defense against bacterial infection in teleost. |
| 资助项目 | National Natural Science Foundation of China[31972831] ; National Key Research and Development Program of China[2018YFD0900501] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000767597600001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178173]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Mo-fei |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Inst Oceanol, Ctr Ocean Mega Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Meng,Jia, Bei-bei,Li, Mo-fei. Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity[J]. FRONTIERS IN IMMUNOLOGY,2022,13:14. |
| APA | Wu, Meng,Jia, Bei-bei,&Li, Mo-fei.(2022).Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity.FRONTIERS IN IMMUNOLOGY,13,14. |
| MLA | Wu, Meng,et al."Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity".FRONTIERS IN IMMUNOLOGY 13(2022):14. |
入库方式： OAI收割
来源：海洋研究所
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