Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands
文献类型：期刊论文
| 作者 | Liu, Xiaojie1,2,3 ; Zhao, Jin2,3; Jiang, Peng2,3
|
| 刊名 | MICROORGANISMS
 |
| 出版日期 | 2022-02-01 |
| 卷号 | 10期号:2页码:8 |
| 关键词 | associated bacteria seaweed silica sand surface sterilization Ulva |
| DOI | 10.3390/microorganisms10020476 |
| 通讯作者 | Jiang, Peng(jiangpeng@qdio.ac.cn) |
| 英文摘要 | Macroalgae-associated bacteria play an important role in their algal hosts' biological processes. They are localized on surfaces of the host thalli, as well as between and even within algal cells. To examine the differences in community structures and functions between epi- and endo- bacteria, an effective approach for maximizing epiphyte removal from delicate seaweeds while retaining endophyte fidelity must be developed. In this study, a variety of surface sterilization methods for Ulva prolifera were compared, including mechanical, chemical, and enzymatical treatments. According to the results of scanning electron microscope (SEM) and denaturing gradient gel electrophoresis (DGGE) analysis, almost complete removal of epiphytic bacteria on Ulva was obtained simply by co-vortex of seaweeds with silica sands, causing minimal disturbance to endosymbionts when compared to previous published methods. In addition, the adaptability was also confirmed in additional U. prolifera strains and Ulva species with blade-like or narrow tubular thallus shapes. This easy mechanical method would enable the analysis of community composition and host specificity for Ulva-associated epi- and endo-bacteria separately. |
| 资助项目 | National Key R&D Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41776153] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019Q05] ; Key R&D Program of Shandong Province[2019GSF107012] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42030302] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000764421500001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178179]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Jiang, Peng |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaojie,Zhao, Jin,Jiang, Peng. Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands[J]. MICROORGANISMS,2022,10(2):8. |
| APA | Liu, Xiaojie,Zhao, Jin,&Jiang, Peng.(2022).Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands.MICROORGANISMS,10(2),8. |
| MLA | Liu, Xiaojie,et al."Easy Removal of Epiphytic Bacteria on Ulva (Ulvophyceae, Chlorophyta) by Vortex with Silica Sands".MICROORGANISMS 10.2(2022):8. |
入库方式： OAI收割
来源：海洋研究所
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