A Deep-Sea Bacterium Senses Blue Light via a BLUF-Dependent Pathway
文献类型：期刊论文
| 作者 | Shan, Yeqi1,2,3,4,5,6; Liu, Ge1,2,3,4,5; Cai, Ruining1,2,3,4,5,6; Liu, Rui1,2,3,4,5; Zheng, Rikuan1,2,3,4,5 ; Sun, Chaomin1,2,3,4,5,6
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| 刊名 | MSYSTEMS
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| 出版日期 | 2022 |
| 卷号 | 7期号:1页码:15 |
| 关键词 | deep sea nonphotosynthetic bacterium blue light BLUF light sensing |
| ISSN号 | 2379-5077 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
| 英文摘要 | Light is a ubiquitous energy source and environmental signal that broadly impacts the lifestyle of a large number of photosynthetic/nonphotosynthetic microorganisms living in the euphotic layer. However, the responses of deep-sea microbes to light are largely unknown, even though blue light is proposed to be distributed in the deep ocean. Here, we successfully cultured a novel bacterial species, named Spongiibacter nanhainus CSC3.9, from deep-sea cold seep samples by a blue light induction approach. The growth of strain CSC3.9 was obviously promoted by the illumination of blue light. We next determined BLUF (a typical blue light photo-receptor) was the most essential factor directing light sensing of strain CSC3.9 through a combined proteomic and genetic method. The function of light sensing mediated by BLUF was further confirmed by the in vitro-synthesized protein. Notably, homologs of BLUF widely existed across the marine microorganisms (containing Spongiibacter species) derived from different environments, including cold seeps. This strongly indicates that the distribution of light utilization by the nonphototrophic bacteria living in the ocean is broad and has been substantially underestimated. IMPORTANCE Extensive studies have been conducted to explore the mechanisms of light sensing and utilization by microorganisms that live in the photic zone. Strikingly, accumulated evidence shows that light is distributed in the deep biosphere. However, the existence and process of light sensing and utilization by microbes inhabiting the deep ocean have been seldom reported. In the present study, a novel bacterial strain, Spongiibacter nanhainus CSC3.9, was enriched and purified from a deep-sea cold seep sample through a blue light induction method. Combined with genomic, proteomic, genetic, and biochemical approaches, the mechanism of this novel strain sensing blue light through a BLUF-dependent pathway was detailed. Our study provides a good model to study the mechanisms of light sensing mediated by deep-sea nonphototrophic bacteria. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Major Research Plan of the National Natural Science Foundation[92051107] ; Key Deployment Projects of Center of OceanMega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000760939200011 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178215]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Marine Biol, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shan, Yeqi,Liu, Ge,Cai, Ruining,et al. A Deep-Sea Bacterium Senses Blue Light via a BLUF-Dependent Pathway[J]. MSYSTEMS,2022,7(1):15. |
| APA | Shan, Yeqi,Liu, Ge,Cai, Ruining,Liu, Rui,Zheng, Rikuan,&Sun, Chaomin.(2022).A Deep-Sea Bacterium Senses Blue Light via a BLUF-Dependent Pathway.MSYSTEMS,7(1),15. |
| MLA | Shan, Yeqi,et al."A Deep-Sea Bacterium Senses Blue Light via a BLUF-Dependent Pathway".MSYSTEMS 7.1(2022):15. |
入库方式： OAI收割
来源：海洋研究所
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