One-Step and Colorimetric Detection of Fish Freshness Indicator Hypoxanthine Based on the Peroxidase Activity of Xanthine Oxidase Grade I Ammonium Sulfate Suspension
文献类型：期刊论文
| 作者 | Guo, Chen2; You, Shuhan2; Li, Changmei2; Chen, Tiantian1 ; Wang, Xiudan2
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| 刊名 | FRONTIERS IN MICROBIOLOGY
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| 出版日期 | 2021-12-02 |
| 卷号 | 12页码:8 |
| 关键词 | hypoxanthine fish freshness colorimetric one-step detection XOD-ASS |
| DOI | 10.3389/fmicb.2021.791227 |
| 通讯作者 | Chen, Tiantian(wangxiudan@qust.edu.cn) ; Wang, Xiudan(chentian0819@163.com) |
| 英文摘要 | The global food waste problem, especially aquatic product spoilage, stimulates the accurate freshness analysis of food products. However, it still remains a great challenge to realize in-field determination of fish freshness at the time of use. In the present study, a colorimetric enzyme biosensor was developed for one-step detection of hypoxanthine (Hx), which is an important intermediate of adenosine triphosphate decomposition during fish storage. We demonstrate that xanthine oxidase grade I ammonium sulfate suspension (XOD-ASS) possesses peroxidase activity. It can oxidize different peroxidase substrates, including 3,3 ',5,5 '-tetramethylbenzidine, 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and o-phenylenediamine in the presence of H2O2, producing visible color reactions. Further experiments indicate that XOD-ASS displayed effective peroxidase activity and could be used for H2O2 detection. Based on this, a one-step Hx detection method was established using only XOD-ASS as the catalyst. The method displays a good linear relationship in the range from 20 to 100 mu M with a detection limit of 6.93 mu M. Additionally, we successfully applied this method in testing Hx accumulation in sea bass fish samples of different storage times. The recovery values range from 97.44 to 102.56%. It is exciting to note that, compared with other methods, our proposed method provides a robust advantage on the economic reaction system, ease of preparation, short time consumption, and moderate reaction temperature. We believe that this method shows good application prospects for on-site fish freshness determination. |
| 资助项目 | National Key Research and Development Program of China[2019YFD0901705] ; Ministry of Science and Technology of the People's Republic of China |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000730264400001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/177518]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Tiantian; Wang, Xiudan |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Chen,You, Shuhan,Li, Changmei,et al. One-Step and Colorimetric Detection of Fish Freshness Indicator Hypoxanthine Based on the Peroxidase Activity of Xanthine Oxidase Grade I Ammonium Sulfate Suspension[J]. FRONTIERS IN MICROBIOLOGY,2021,12:8. |
| APA | Guo, Chen,You, Shuhan,Li, Changmei,Chen, Tiantian,&Wang, Xiudan.(2021).One-Step and Colorimetric Detection of Fish Freshness Indicator Hypoxanthine Based on the Peroxidase Activity of Xanthine Oxidase Grade I Ammonium Sulfate Suspension.FRONTIERS IN MICROBIOLOGY,12,8. |
| MLA | Guo, Chen,et al."One-Step and Colorimetric Detection of Fish Freshness Indicator Hypoxanthine Based on the Peroxidase Activity of Xanthine Oxidase Grade I Ammonium Sulfate Suspension".FRONTIERS IN MICROBIOLOGY 12(2021):8. |
入库方式： OAI收割
来源：海洋研究所
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