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A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response

文献类型：期刊论文

作者Zou, Guangyue2; Chen, Shuai2; Liu, Nazhen1; Yu, Yongliang2
刊名CHINESE CHEMICAL LETTERS
出版日期2022-02-01
卷号33期号:2页码:778-782
关键词Carbon dots Polarity Lysosome Ratiometric sensing Wide linear range
ISSN号1001-8417
DOI10.1016/j.cclet.2021.08.076
通讯作者Liu, Nazhen(liunazhen@qdio.ac.cn) ; Yu, Yongliang(yuyl@mail.neu.edu.cn)
英文摘要Lysosomal polarity is considered a key indicator of lysosomal function due to its significant impact on membrane fluidity and enzymatic reactions in lysosomes. Monitoring lysosomal polarity can gain insight into the related physiological and pathological processes and develop new diagnostic methods. However, current fluorescent probes with lysosomal polarity response suffer from narrow linear range, photo-bleaching and complicated preparation. Herein, a ratiometric fluorescent probe (r-bCDs) for intracellular lysosomal polarity imaging is designed and constructed by amide bond assembly of polarity-sensitive red fluorescent carbon dots (rCDs) and referenced blue fluorescent carbon dots (bCDs). r-bCDs show a much wider linear range of polarity response (orientation polarizability Delta f from 0.020 to 0.315) than other probes, and the interference of uneven distribution and instrument factors can be effectively eliminated by ratiometric fluorescent sensing. Imaging of intracellular lysosomal polarity with r-bCDs is implemented to observe the polarity variation caused by the change of cell state and the difference between cancer cells and normal cells. This work provides a promising tool for studying the related physiological and pathological processes and developing new diagnostic methods. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
资助项目National Natural Science Foundation of China[21922402] ; National Natural Science Foundation of China[21874017] ; National Natural Science Foundation of China[21727811] ; Fundamental Research Funds for the Central Universities[N2005027]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000760891400023
出版者ELSEVIER SCIENCE INC
源URL[http://ir.qdio.ac.cn/handle/337002/178182]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Liu, Nazhen; Yu, Yongliang
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Shenyang 110819, Peoples R China
推荐引用方式
GB/T 7714		 Zou, Guangyue,Chen, Shuai,Liu, Nazhen,et al. A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response[J]. CHINESE CHEMICAL LETTERS,2022,33(2):778-782.
APA Zou, Guangyue,Chen, Shuai,Liu, Nazhen,&Yu, Yongliang.(2022).A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response.CHINESE CHEMICAL LETTERS,33(2),778-782.
MLA Zou, Guangyue,et al."A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response".CHINESE CHEMICAL LETTERS 33.2(2022):778-782.

入库方式： OAI收割

来源：海洋研究所

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