Isolation, structural modification, characterization, and bioactivity of polysaccharides from Folium Isatidis
文献类型:期刊论文
作者 | Abuduwaili, A (Abuduwaili, Aytursun) 1 , 2; Nuerxiati, R (Nuerxiati, Rehebati) 1 , 2; Mutailifu, P (Mutailifu, Paiheerding) 1 , 2; Gao, YH (Gao, Yanhua) 1; Lu, CF (Lu, Chunfang) 1; Yili, A (Yili, Abulimiti) 1 |
刊名 | INDUSTRIAL CROPS AND PRODUCTS |
出版日期 | 2022 |
卷号 | 176期号:2页码:1-14 |
ISSN号 | 0926-6690 |
关键词 | Folium Isatidis Polysaccharide Structural modification |
DOI | 10.1016/j.indcrop.2021.114319 |
英文摘要 | The purification process of Folium Isatidis polysaccharide (FIP) was optimized by response surface method (RSM) using AB-8 macroporous resin. A major homogeneous polysaccharide, named FIP-II, was purified from FIP through DEAE-650 M and Sephadex G-75 column chromatography. The structure of the FIP-II was investigated through the combination of HPLC, GC, FT-IR, sequential acid hydrolysis, peroxide oxidation, Smith degradation, and NMR analysis. Then, the sulfated, phosphorylated, and carboxymethylated derivatives of FIP-II, namely FIPII-S, FIP-II-P, and FIP-II-C, were prepared. Their structures were characterized by extensive analysis of chromatographic and spectroscopic methods including UV, HPLC, GC, FT-IR, Congo red, CD, SEM-EDX, XRD, TG, DTG, and DSC. Results of RSM showed that optimum purification conditions were elution flow rate 2.76 BV/ h, the sample concentration 2.03 mg/mL, and the elution volume was 1.26 BV. The average comprehensive score of purification was up to 76.28%. FIP-II and its derivatives were heteropolysaccharides composed of different molar ratios of rhamnose, arabinose, xylose, mannose, glucose, and galactose, with a molecular weight of 775.64, 911.75, 1288.31, and 1013.61 kDa. The structure modification influences the solution conformation, surface morphology, crystalline nature, and thermal properties of the FIP-II and its derivatives. Antioxidant activity tests showed that structure modification could significantly enhance the activity of the polysaccharides differently. It could be a potential ingredient in the functional food and pharmaceutical industry. |
WOS记录号 | WOS:000736982000004 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/8132] |
专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
通讯作者 | Yili, A (Yili, Abulimiti) 1 |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China |
推荐引用方式 GB/T 7714 | Abuduwaili, A ,Nuerxiati, R ,Mutailifu, P ,et al. Isolation, structural modification, characterization, and bioactivity of polysaccharides from Folium Isatidis[J]. INDUSTRIAL CROPS AND PRODUCTS,2022,176(2):1-14. |
APA | Abuduwaili, A ,Nuerxiati, R ,Mutailifu, P ,Gao, YH ,Lu, CF ,&Yili, A .(2022).Isolation, structural modification, characterization, and bioactivity of polysaccharides from Folium Isatidis.INDUSTRIAL CROPS AND PRODUCTS,176(2),1-14. |
MLA | Abuduwaili, A ,et al."Isolation, structural modification, characterization, and bioactivity of polysaccharides from Folium Isatidis".INDUSTRIAL CROPS AND PRODUCTS 176.2(2022):1-14. |
入库方式: OAI收割
来源:新疆理化技术研究所
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