A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales
文献类型：期刊论文
| 作者 | Li, XN; Sun, HH; Zhang, GF; Bi, JX; Ma, GH; Ma, RY; Su, ZG |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2005-11-01 |
| 卷号 | 28期号:17页码:2363-2369 |
| 关键词 | cyanopropyl stationary phase huperzine A huperzine B purification resolution |
| ISSN号 | 1615-9306 |
| 英文摘要 | Huperzine A (HupA) and huperzine B (HupB) are two medically important components of Huperzia serrata. It is difficult to obtain high yields of the separation from the plant using conventional liquid extraction and chromatography. However, this study has found that RP chromatography with cyanopropyl (CN) medium was able to separate these two analogs simultaneously from the plant extract with higher resolution. Comparison between a CN medium and a popular C18 medium demonstrated the superiority of the CN over the C18 in resolution for both analytical and preparative separation of HupA and HupB. A preparative process was developed for simultaneous purification of HupA and HupB from H. serrata. The yields on the basis of the mass of the herbal powder for HupA and HupB were 0.019 and 0.008% respectively, which were about 1.9 and 10 times of those reported in the literature. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; ENANTIOSELECTIVE SYNTHESIS ; (-)-HUPERZINE-A ; INHIBITOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000233679200018 |
| 公开日期 | 2013-09-20 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1578]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Beijing Univ Chem Technol, Coll Life Sci, Dept Pharmaceut Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Lab Biochem Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, XN,Sun, HH,Zhang, GF,et al. A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales[J]. JOURNAL OF SEPARATION SCIENCE,2005,28(17):2363-2369. |
| APA | Li, XN.,Sun, HH.,Zhang, GF.,Bi, JX.,Ma, GH.,...&Su, ZG.(2005).A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales.JOURNAL OF SEPARATION SCIENCE,28(17),2363-2369. |
| MLA | Li, XN,et al."A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales".JOURNAL OF SEPARATION SCIENCE 28.17(2005):2363-2369. |
入库方式： OAI收割
来源：过程工程研究所
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