Application of response surface methodology to the modeling of alpha-amylase purification by aqueous two-phase systems
文献类型：期刊论文
| 作者 | Zhi, WB; Song, JN; Ouyang, F; Bi, JX |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2005-08-04 |
| 卷号 | 118期号:2页码:157-165 |
| 关键词 | alpha-amylase aqueous two-phase systems purification response surface methodology |
| ISSN号 | 0168-1656 |
| 英文摘要 | Mathematical models concerning the purification of alpha-amylase from the cultivation supernatant of Bacillus subtilis in a polyethylene glycol-citrate aqueous two-phase system (ATP) are established with response surface methodology. The PEG3350, citrate and sodium chloride concentrations were selected as variables to evaluate the purification impact factors in aqueous two-phase system, including partition coefficients of alpha-amylase, total protein, purification factor and alpha-amylase yield. An experimental space with two-fold purification and over 90% yield of alpha-amylase is achieved through the optimized condition basing on the model. Two systems with low viscosity within said space were further selected to perform alpha-amylase purification and the experimental results coincide well with the calculation of the models, which indicates that the model provides a promising tool for experimental design of protein purification by aqueous two-phase system. (C) 2005 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | OPTIMIZATION ; EXTRACTION ; PARTITION ; SEPARATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230580300004 |
| 公开日期 | 2013-09-20 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1590]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhi, WB,Song, JN,Ouyang, F,et al. Application of response surface methodology to the modeling of alpha-amylase purification by aqueous two-phase systems[J]. JOURNAL OF BIOTECHNOLOGY,2005,118(2):157-165. |
| APA | Zhi, WB,Song, JN,Ouyang, F,&Bi, JX.(2005).Application of response surface methodology to the modeling of alpha-amylase purification by aqueous two-phase systems.JOURNAL OF BIOTECHNOLOGY,118(2),157-165. |
| MLA | Zhi, WB,et al."Application of response surface methodology to the modeling of alpha-amylase purification by aqueous two-phase systems".JOURNAL OF BIOTECHNOLOGY 118.2(2005):157-165. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。