Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: Characteristics and effects on rats
文献类型：期刊论文
| 作者 | Lu, XL; Zheng, CY; Shi, XD; Wang, YQ; Suo, XY; Yu, PZ; Xu, YH; Ma, TM; Su, ZG |
| 刊名 | ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 33期号:2页码:83-99 |
| 关键词 | bovine hemoglobin human serum albumin conjugate blood substitutes glutaraldehyde |
| ISSN号 | 1073-1199 |
| 英文摘要 | Conjugate of bovine hemoglobin (bHb) and human serum albumin (HSA) was prepared. The product was simply composed of 89.7% one-to-one Hb-HSA conjugate, 6.0% oligomer of Hb and HSA, 3.5% unconjugated HSA and 0.8% unconjugated Hb, with an average molecular weight of 157kD. The physicochemical characteristics were determined. Effects of single replacement on blood pressure and long-term survival of rats with 30% and 60% acute blood loss were studied, in comparison with Ringer-lactate solution, stroma-free hemoglobin (SFHb), 5% HSA in Ringer-lactate, whole blood and no resuscitation fluid. Results showed that Hb-HSA conjugate maintained the mean arterial pressure of rats to initial level with no pressor effect. Long-term effects of the replacement fluids on 30% bleeding rats showed that, for the group infused with Hb-HSA conjugate, histology of five major organs, heart, kidney, liver, spleen and lung, were essentially normal, similar to that of whole blood, while obviously renal side-effects appeared in other groups. The efficacy of the conjugate was further demonstrated by the resuscitation of lethal hemorrhagic shock rats (60% acute blood loss) with 100% survival rate (followed for 14 days), the same result as whole blood. The Hb-HSA conjugate can thus be another candidate for blood substitute in emergency. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| 关键词[WOS] | CROSS-LINKED HEMOGLOBIN ; SAFETY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230198300001 |
| 公开日期 | 2013-09-20 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1603]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Univ Sci & Technol, Dept Environm Engn, Beijing, Peoples R China 3.Beijing Univ Chem Technol, Dept Bioengn, Beijing, Peoples R China 4.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, XL,Zheng, CY,Shi, XD,et al. Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: Characteristics and effects on rats[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2005,33(2):83-99. |
| APA | Lu, XL.,Zheng, CY.,Shi, XD.,Wang, YQ.,Suo, XY.,...&Su, ZG.(2005).Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: Characteristics and effects on rats.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,33(2),83-99. |
| MLA | Lu, XL,et al."Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: Characteristics and effects on rats".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 33.2(2005):83-99. |
入库方式： OAI收割
来源：过程工程研究所
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